|
| 產地 | 進口、國產 |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS12384 |
| 應用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 IF=1:50-200 |
| CAS編號 | |
| 抗體名 | Anti-SGK3 |
| 克隆性 | 是 |
| 靶點 | 詳見說明書 |
| 適應物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human SGK3 |
技術外包服務:
★絲氨酸/蘇氨酸蛋白激酶Sgk3抗體說明書 分子生物學：質粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構建等
★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構建、細胞RNAi技術等等。
實驗流程：
絲氨酸/蘇氨酸蛋白激酶Sgk3抗體說明書 英文名稱 Anti-SGK3
中文名稱 絲氨酸/蘇氨酸蛋白激酶Sgk3抗體說明書
別 名 CISK; Cytokine independent survival kinase; Serine/threonine protein kinase Sgk3; Serine/threonine-protein kinase Sgk3; Serum/glucocorticoid regulated kinase 3; Serum/glucocorticoid regulated kinase family member 3; Serum/glucocorticoid regulated kinase like; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like; SGK 2; SGK 3; SGK2; Sgk3; SGK3_HUMAN; SGKL.
產品屬性：
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
產品類型 一抗
研究領域 細胞生物 免疫學 信號轉導 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 48kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SGK3
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
絲氨酸/蘇氨酸蛋白激酶Sgk3抗體說明書 產品應用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 IF=1:50-200
（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 Serine/threonine-protein kinase Sgk3 (SGK3), also designated serum/glucocorticoid regulated kinase 3, belongs to the Ser/Thr protein kinase family of proteins. The serum- and glucocorticoid-regulated kinase proteins are closely related to the Akt protein family. SGK1, a homolog of SGK3, activates ion channels, in particular potassium (K+) channels. SGK2 and SGK3 have been found to also be involved in this activation process, making all three of these proteins important regulators for cell proliferation, epithelial transport and neuromuscular excitability. SGK3 acts as a mediator of IL-3 dependent survival signals in the cell. It localizes to the early endosome and in vesicle-like structures. SGK3 is a widely expressed protein, but it is primarily detected in kidney, liver, pancreas, brain and heart. Phosphorylation of SGK3 at residue Ser 486 leads to an increase in SGK3 activation.
Function : Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.
Subunit : Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner.
Subcellular Location : Cytoplasmic vesicle. Early endosome. Localized in vesicle-like structures and in the early endosome.
Tissue Specificity : Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle.
Post-translational modifications : Activated by phosphorylation on Ser-486 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 PX (phox homology) domain.
Database links : UniProtKB/Swiss-Prot: Q96BR1.1
全、新、優(yōu)、品、好四大特點：
絲氨酸/蘇氨酸蛋白激酶Sgk3抗體說明書 全：公司提供上萬種產品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產品在我司都能找到。
新：產品更新速度較快，基本上每周都有新產品出現。
優(yōu)：產品質量好，投訴比較少。
好：我公司具有優(yōu)質的技術團隊，產品一旦售出，實驗過程中遇到困難可提供在線技術咨詢。使您使用產品時沒有任何的后顧之憂。
正在熱銷的相關產品：
改良甘露醇卵黃多粘菌素瓊脂基礎250g用于蠟樣芽孢桿菌分離培養(yǎng)和計數
心浸液瓊脂 Heart Infusion Agar 用于培養(yǎng)營養(yǎng)要求高的微生物
麥康凱瓊脂3號 Maconkey Agar No.3 250 用于腸道致病菌的選擇性分離、培養(yǎng)(OXOID方法)
沙氏葡萄糖瓊脂培養(yǎng)基250g/瓶用于真菌培養(yǎng)和檢測incubationmedia沙氏葡萄糖瓊脂培養(yǎng)基250g/瓶用于真菌培養(yǎng)和檢測
LST-MUG
改良克氏雙糖培養(yǎng)基2990g用于小腸耶爾森氏菌的鑒定
3231-prf SAEpiCM-prf 小氣道上皮細胞培養(yǎng)基 500 ml incubation media 3231-prf SAEpiCM-prf 小氣道上皮細胞培養(yǎng)基 500 ml
玫瑰紅瓊脂培養(yǎng)基 300ml/袋*10 用于霉菌、酵母菌計數
腸球菌瓊脂(膽汁七葉苷瓊脂)250g用于食品和水中腸球菌的計數。
EugonicAgar
EB增菌液凍干配套試劑SR0090支添加于225ml(0260成EB增菌液。
Mueller-Hinton 瓊脂 (CM0337) Oxoid incubation media Mueller-Hinton 瓊脂 (CM0337) Oxoid
繩狀青霉 溶菌試驗 支/瓶
西蒙氏枸櫞酸鹽瓊脂250g用于腸道菌的檸檬酸鹽利用試驗(GB標準)
AgarmediumK(Xylose,lysine,deoxycholateagar)
CL-0138Lec1(倉鼠卵巢細胞)5×106cells/瓶×2
BDNF Others Mouse 小鼠 / 人 BDNF CHO細胞裂解液 (陽性對照)
人骨骼肌衛(wèi)生細胞裂解物HSkMSCL
貂源細胞 淋巴細胞細胞，A3細胞 3T3-swiss albino細胞，小鼠胚胎成纖維細胞
人胚腎細胞;293 Cells, low passage
HA Others H1N1 甲型 H1N1 (A/New Caledonia/20/1999) 血凝素HA1 (Hemagglutinin) 人細胞裂解液 (陽性對照)
絲氨酸/蘇氨酸蛋白激酶Sgk3抗體說明書CL-0136L6(大鼠成肌細胞)5×106cells/瓶×2
GPT Others Rat 大鼠 GPT1 / GPT 桿狀病毒-昆蟲細胞裂解液 (陽性對照)
人氣管平滑肌細胞裂解物HTSMCL
HPAC細胞，人*癌細胞 人上皮細胞,16HBE細胞 皮膚肥大細胞Many types of cells包裝：5 × 105次方(1ml)
兔眼Tenon's囊成纖維細胞;RYTF
TGFBR2 Others Human 人 TGFβR2 / TGFR2 人細胞裂解液 (陽性對照)
