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| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS12368 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-SNAI2/SLUG |
| 克隆性 | 是 |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human Slug N-terminus |
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 鋅指轉(zhuǎn)錄因子Slug抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
產(chǎn)品訂購(gòu)信息：
英文名稱 Anti-SNAI2/SLUG
中文名稱 鋅指轉(zhuǎn)錄因子Slug抗體說明書
別 名 Neural crest transcription factor Slug; Protein snail homolog 2; Slug (chicken homolog) zinc finger protein; Slug homolog zinc finger protein; Slug zinc finger protein; SLUGH 1; SLUGH; SLUGH1; SNAI 2; SNAI2; SNAI-2; SNAI2_HUMAN; Snail 2; Snail homolog 2; Snail2; Snail-2; WS 2D; WS2D; Zinc finger protein SLUG; Zinc finger protein SNAI2.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子
蛋白分子量 predicted molecular weight: 30kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Slug N-terminus
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
鋅指轉(zhuǎn)錄因子Slug抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008].
Function : Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occluding (OCLN) and subsequent oncogenic transformation of epithelial cells.
Subunit : Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).
Subcellular Location : Nucleus.
Tissue Specificity : Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
DISEASE : Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) . WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1
Similarity : Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Database links : UniProtKB/Swiss-Prot: O43623.1
轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators）
鋅指轉(zhuǎn)錄因子Slug主要用于轉(zhuǎn)移方面的研究。
抗體的鑒定：
1）鋅指轉(zhuǎn)錄因子Slug抗體說明書 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
改良山梨醇麥康凱瓊脂基礎(chǔ)(CT-SMAC)2550g用于大腸埃希氏菌07/NM的選擇性分離培養(yǎng)(GB/T4789.36-2008)。
Peptone-D (Protease peptone) 蛋白胨 D 100g incubation media Peptone-D (Protease peptone) 蛋白胨 D 100g
謝氏桿菌 支/瓶
葡萄糖瓊脂250g用于細(xì)菌的綜合生化試驗(yàn)
精氨酸肉湯 250g 用于游泳池中假單胞菌檢測(cè)(Merck方法)
改良纖維二糖多粘菌素B多粘菌素E(mCPC)瓊脂基礎(chǔ)凍干配套試劑SR0260支添加于50ml025纖維二糖多粘菌素B多粘菌素E(mCPC)瓊脂基礎(chǔ)。
LB Lennox Broth 培養(yǎng)基 250g incubation media LB Lennox Broth 培養(yǎng)基 250g
煙曲霉 支/瓶
桔汁瓊脂250g用于飲料中耐酸細(xì)菌的分離培養(yǎng)
溶血(0.1%)赫氏瓊脂 250g 用于菌分離培養(yǎng)
3%酶試劑2ml*20支用于細(xì)菌的酶試驗(yàn)
MRS瓊脂 MRS Agar Base 用于食品中乳酸菌總數(shù)測(cè)定
XLD瓊脂培養(yǎng)基 Xylose Lysine Desoxycholate Medium 100克 分離志賀氏菌屬和沙門氏菌
胰蛋白胨瓊脂培養(yǎng)基250g/瓶用于布魯氏菌屬檢驗(yàn)incubationmedia胰蛋白胨瓊脂培養(yǎng)基250g/瓶用于布魯氏菌屬檢驗(yàn)
BileEsculinAgar
CL-0170NG108-15(小鼠神經(jīng)細(xì)胞瘤與大鼠神經(jīng)之融合細(xì)胞)5×106cells/瓶×2
RSPO1 Others Mouse 小鼠 R-Spondin 1 / RSPO1 CHO細(xì)胞裂解液 (陽性對(duì)照)
人虹膜色素上皮細(xì)胞裂解物HIPEpiCL
小耳豬肺細(xì)胞;SEP-L2 大細(xì)胞細(xì)胞，NCI-H661細(xì)胞 DSL-6A/C1細(xì)胞，大鼠*癌細(xì)胞株
人整合SV40基因的上皮細(xì)胞;HBL-100 [HBL100]
HA Others H1N1 甲型 H1N1 (A/Ohio/UR06-0091/2007) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽性對(duì)照)
鋅指轉(zhuǎn)錄因子Slug抗體說明書CL-0168Neuro-2a/N2a(小鼠腦神經(jīng)瘤細(xì)胞)5×106cells/瓶×2
GSK3B Others Human 人 GSK3B 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對(duì)照)
人視網(wǎng)膜色素上皮細(xì)胞裂解物HRPEpiCL
NCI-H1755[H1755]細(xì)胞，人細(xì)胞 人胚,HELF細(xì)胞 卵巢上皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
兔主動(dòng)脈平滑肌細(xì)胞;SMC
TIMP2 Others Human 人 TIMP2 / TIMP-2 人細(xì)胞裂解液 (陽性對(duì)照)
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