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產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS12089 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-SMC6 |
克隆性 | 是 |
靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
宿主 | 詳見(jiàn)說(shuō)明書(shū) |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human SMC6 |
產(chǎn)品訂購(gòu)信息:
英文名稱(chēng) Anti-SMC6
中文名稱(chēng) 染色體結(jié)構(gòu)維持蛋白6抗體費(fèi)用
別 名 SMC6L1; SMC 6; SMC6; SMC6 protein; SMC6 structural maintenance of chromosomes 6 like 1; Structural maintenance of chromosomes 6; Structural maintenance of chromosomes 6 like 1; Structural maintenance of chromosomes protein 6.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類(lèi)型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep
產(chǎn)品類(lèi)型 一抗
研究領(lǐng)域 細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化 表觀(guān)遺傳學(xué)
蛋白分子量 predicted molecular weight: 126 kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SMC6
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
染色體結(jié)構(gòu)維持蛋白6抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 SMC5/6 complex is recruited to nuclease induced double strand breaks (DSBs) and is required for the recruitment of cohesin to DSBs. The human SMC5/6 complex and the SUMO ligase activity of hMMS21 are required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells.
Function : Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies.
Subunit : Forms a heterodimer with SMC5. Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NDNL2.
Subcellular Location : Cytoplasm. Nucleus.
Tissue Specificity : Widely expressed. Overexpressed in testis.
Post-translational modifications : Ubiquitinated.
Similarity : Belongs to the SMC family. SMC6 subfamily.
Database links : UniProtKB/Swiss-Prot: Q96SB8.2
Anti-Phospho-HSL (Ser565) /FITC 熒光素標(biāo)記磷酸化荷爾蒙敏感脂肪酶抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-PI3 Kinase p110 delta/FITC 熒光素標(biāo)記磷脂酰肌醇激酶催化亞單位D抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh ASMTL ASMTL蛋白抗體 規(guī)格 0.2ml
Donkey Anti-Guinea pig IgG/Alexa Fluor 488 Alexa Fluor 488標(biāo)記的驢抗豚鼠IgG 0.1ml
Glucocorticoid Receptor beta 英文名稱(chēng): 糖皮質(zhì)激素受體β抗體 0.2ml
Rhesus antibody Rh Rabbit Anti-mouse IgG/HRP 辣根過(guò)氧化物酶標(biāo)記的兔抗小鼠IgG 規(guī)格 0.1ml
Anti-PI3 Kinase p110 delta/FITC 熒光素標(biāo)記磷脂酰肌醇激酶催化亞單位D抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-Dnmt1/FITC 熒光素標(biāo)記DNA甲基轉(zhuǎn)移酶1抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-MCM2/FITC 熒光素標(biāo)記染色體維持缺陷蛋白2抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh CABLES2 細(xì)胞周期蛋白依賴(lài)性激酶CABLES2抗體 規(guī)格 0.2ml
CLCA4(Calcium-activated chloride channel 4) 鈣激活氯離子通道4抗原 0.5mg
HOXB8 英文名稱(chēng): 同源盒蛋白B8抗體 0.1ml
Rhesus antibody Rh SEMA4D/CD100 臂板蛋白4D抗體 規(guī)格 0.2ml
Anti-MCM2/FITC 熒光素標(biāo)記染色體維持缺陷蛋白2抗體IgGMulti-class antibodies規(guī)格: 0.2ml
PYD(Human Pyridinoline crosslinks) ELISA Kit 人膠原吡啶交聯(lián)Multi-class antibodies規(guī)格: 48T
Anti-MCP-3/CCL7 單核細(xì)胞趨化蛋白3抗體Multi-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh KAL1 卡爾曼綜合征基因1抗體 規(guī)格 0.2ml
TGF- Alpha ELISA Kit 大鼠轉(zhuǎn)化生長(zhǎng)因子α 96T
phospho-PRKCQ(Ser676) 英文名稱(chēng): 磷酸化蛋白激酶C theta抗體 0.1ml
CD26/DPP4 英文名稱(chēng): CD26抗體 0.1ml
Anti-MCP-3/CCL7 單核細(xì)胞趨化蛋白3抗體Multi-class antibodies規(guī)格: 0.2ml
IL1R1 Others Cynomolgus 食蟹猴 IL1R1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人胃腺癌細(xì)胞;BGC-823
CM-H130人視網(wǎng)膜微內(nèi)皮細(xì)胞完全培養(yǎng)基100mL
MKN-28細(xì)胞,人細(xì)胞 人細(xì)胞,U-2 OS細(xì)胞 幼蚊細(xì)胞;C6/36
DLD-1(人腺癌細(xì)胞) 5×106cells/瓶×2
CA9 Others Human 人 CA9 / CAIX 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
CL-0424RK1(大鼠腎細(xì)胞)5×106cells/瓶×2
ICOSLG Others Human 人 ICOS Ligand / B7-H2 / ICOSLG 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人內(nèi)根殼細(xì)胞RNAHHIRSC miRNA5 μg
QBC939細(xì)胞,人細(xì)胞 褐鼠瘤上皮細(xì)胞,RIN-m細(xì)胞 NIH/3T3, 小鼠成纖維細(xì)胞系
MA-891(小鼠癌高轉(zhuǎn)移細(xì)胞) 5×106cells/瓶×2
NHEK adult pooled Pellet 正常人類(lèi)表皮角質(zhì)細(xì)胞團(tuán)塊(捐獻(xiàn)者,混合來(lái)源) > 1 mio.cells 雪旺細(xì)胞培養(yǎng)基SCM
染色體結(jié)構(gòu)維持蛋白6抗體費(fèi)用 CL-0422RBL-1(大鼠嗜堿性粒細(xì)胞性細(xì)胞)5×106cells/瓶×2
CD40LG Others Canine 狗 CD40L / CD154 / TNFSF5 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人基質(zhì)細(xì)胞RNAHHGMC miRNA5 μg
兔腎細(xì)胞;RK-13 細(xì)胞,KG-1細(xì)胞 MFC-GFP細(xì)胞,綠色熒光蛋白標(biāo)記小鼠前細(xì)胞
人類(lèi)成纖維細(xì)胞系;CNLMG-B5537SKIN
HA Others H3N2 甲型 H3N2 (A/Aichi/2/1968) 血凝素 (Hemagglutinin / HA) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 染色體結(jié)構(gòu)維持蛋白6抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件;
3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)染色體結(jié)構(gòu)維持蛋白6抗體費(fèi)用 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線(xiàn),計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。