无码欧美成人av天堂456软件,大胆日本熟妇XXXX,9277在线观看免费播放,4p三个男人让我爽了一夜

產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-phospho-TOPO II Alpha (Ser1106) 

中文名稱   磷酸化DNA拓普西異構(gòu)酶Ⅱ抗體規(guī)格 

別    名  DNA topoisomeraseⅡalpha; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2; TP2A; TOP2A; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA Topoisomerase2; TOP 2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TOP2A_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human 

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 174kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human TOPO II Alpha around the phosphorylation site of Ser1106 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化DNA拓普西異構(gòu)酶Ⅱ抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Function : Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Subunit : Homodimer. Interacts with COPS5.

Subcellular Location : Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm.

Post-translational modifications : Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

Similarity : Belongs to the type II topoisomerase family.

Contains 1 Toprim domain.

Database links : UniProtKB/Swiss-Prot: P11388.3

Integrin Alpha 4/ITGA4(Integrin alpha 4) 整合素-α(抗原)Multi-class antibodies規(guī)格： 0.5ml

Anti-CD24 CD24抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh PAK7/PAK5/MBT 激活激酶PAK7抗體 規(guī)格 0.2ml

EFNA1 Kit Human 人 Ephrin-A1 / EFNA1 ELISA配對(duì)抗體 ELISA

Tropomyosin 英文名稱： 原肌球蛋白1抗體 0.1ml

NLF3 英文名稱： 限局性核因子3抗體 0.2ml

Anti-CD24 CD24抗體Multi-class antibodies規(guī)格： 0.2ml

IL-9 ELISA Kit 大鼠白介素9Multi-class antibodies規(guī)格： 48T

Anti-arfaptin 1/FITC 熒光素標(biāo)記ADP核糖基化因子結(jié)合蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FAM151A FAM151A蛋白抗體 規(guī)格 0.2ml

B7-2/sCD86(Human soluble Cluster of differentiation 86) ELISA Kit 人可溶性CD86 96T

Music Acetylcholine receptor M3 英文名稱： 毒蕈堿型乙酰膽堿受體M3抗體 0.1ml

ANKK1 英文名稱： 錨定蛋白重復(fù)結(jié)構(gòu)域蛋白激酶ANKK1抗體 0.2ml

Anti-arfaptin 1/FITC 熒光素標(biāo)記ADP核糖基化因子結(jié)合蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Thioredoxin 1/FITC 熒光素標(biāo)記硫氧還蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

NKR ; Neurokin B receptor (Neuromedin K receptor; NK-4 receptor) 神經(jīng)激肽-B受體(抗原)Multi-class antibodies規(guī)格： 0.5mg

血影蛋白/收縮蛋白抗體 Anti-Spectrin-α 0.2ml

Semaphorin 3B 英文名稱： 導(dǎo)向蛋白3B抗體 0.2ml

Estrogen Receptor alpha + beta 英文名稱： 雌激素受體α/β抗體 0.1ml

Rhesus antibody Rh phospho-MAP4K1(Ser171) 磷酸化造血祖細(xì)胞激酶抗體 規(guī)格 0.1ml

NKR ; Neurokin B receptor (Neuromedin K receptor; NK-4 receptor) 神經(jīng)激肽-B受體(抗原)Multi-class antibodies規(guī)格： 0.5mg

大鼠腺來(lái)源的內(nèi)皮生長(zhǎng)因子(EG-VEGF)ELISA試劑盒 ，英文名： EG-VEGF ELISA Kit

人抑制因子受體(LIFR)ELISA檢測(cè)試劑盒Humanleukemiainhibitoryfactorreceptor,LIFRELISAKit 96T/48T

魚(yú)5羥色胺/血清素/血清胺(5HT/ST)免疫試劑盒 Fish 5-hydroxyyptamine/serotonin,5HT/ST ELISA kit

CLIAKitforPAPP-A(HumanpregnancyassociatedplasmaproteinA)ELISAKit人相關(guān)血漿蛋白A規(guī)格：48T/96T

水果瓜氨酸含量比色法定量檢測(cè)試劑盒20次

ELISAKitmIgM人表面膜免疫球蛋白M規(guī)格：48T/96T

大鼠成纖維細(xì)胞生長(zhǎng)因子15(FGF15)ELISA試劑盒 ，英文名： FGF15 ELISA Kit

Rat leukemia inhibitory factor receptor (LIFR) ELISA Kit 大鼠抑制因子受體(LIFR)ELISA試劑盒

RatSolubleprotein-100B,S-100BELISAKit 大鼠S100B蛋白(S-100B)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforCRHELISAKit大鼠促腎上皮質(zhì)激素釋放激素規(guī)格：48T/96T

細(xì)胞緊張素Ⅰ轉(zhuǎn)化酶1(ACE1)活性熒光共振能量轉(zhuǎn)移法(FRET)定量檢測(cè)試劑盒20次

RatCytochrome-C,Cyt-CELISAKit大鼠細(xì)胞色素C(Cyt-C)ELISA試劑盒規(guī)格：96T/48T

磷酸化DNA拓普西異構(gòu)酶Ⅱ抗體規(guī)格 大鼠多巴胺(DA)ELISA試劑盒 ，英文名： DA ELISA Kit

Porcine heat shock protein 90 (HSP-90) ELISA Kit 豬熱休克蛋白90(HSP-90)ELISA試劑盒

馬特定基因序列(Hor)核酸檢測(cè)試劑盒(PCR-熒光探針?lè)? 48T

CLIAKitforLTC4(MouseLeukoieneC4)ELISAKit小鼠白三烯C4規(guī)格：48T/96T

體液總巰基含量熒光定量檢測(cè)試劑盒20次

ELISAKitADM雞腎上腺髓質(zhì)素規(guī)格：48T/96T

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酸化DNA拓普西異構(gòu)酶Ⅱ抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）磷酸化DNA拓普西異構(gòu)酶Ⅱ抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。