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原肌球蛋白抗體說明書 英文名稱  Anti-Trop-2

中文名稱  原肌球蛋白抗體說明書 

別    名 AMCD1; Beta tropomyosin muscle; BETA-TM; DA1; DA2B; EPITHELIAL TROPOMYOSIN; FIBROBLAST MUSCLE TYPE TROPOMYOSIN; MGC109519; NEM4; TM2; TMSB; TPM2; TROP-2; TROPOMYOSIN 2; Tropomyosin 2 (beta chain); TPM2_MOUSE .

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 細(xì)胞表面分子 細(xì)胞分化

蛋白分子量  predicted molecular weight: 33kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from mouse Trop-2 C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

原肌球蛋白抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.

Function : Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.

Subunit : Heterodimer of an alpha and a beta chain.

Subcellular Location : Cytoplasm, cytoskeleton.

Post-translational modifications : Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization.

Similarity : Belongs to the tropomyosin family.

Database links : UniProtKB/Swiss-Prot: P58774.1

Trop-2蛋白主要存在于細(xì)胞胞質(zhì)及表面, 它是絲裂原活化蛋白激酶(MAPK)通路的一個重要組成部，參與細(xì)胞生長、增殖、分化、死亡及細(xì)胞間的功能同步等多種生理過程.

Trop-2在很多常見的上扮演著重要角色，其中包括、、、、、*癌和，有學(xué)者認(rèn)為：Trop-2在孕期內(nèi)所具有的生殖功能可能意味著它同的生長有關(guān)聯(lián)。

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