无码欧美成人av天堂456软件,大胆日本熟妇XXXX,9277在线观看免费播放,4p三个男人让我爽了一夜

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. Toll樣受體2抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購(gòu)信息：
英文名稱(chēng)  Anti-TLR2/CD282 

中文名稱(chēng)   Toll樣受體2抗體規(guī)格 

別    名  Toll-like receptor 2; Toll like receptor 2; Toll like receptor 2 precursor; Toll-like receptor 2; TLR 2; TLR2; TLR-2; Toll/interleukin-1 receptor-like protein 4; CD282 antigen; CD282; TIL 4; TIL4; TLR2_HUMAN; Toll/interleukin 1 receptor like 4; Toll/interleukin 1 receptor like protein 4; Toll/interleukin receptor like protein 4.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg 1ml/1mg

抗體來(lái)源   Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep 

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域     免疫學(xué) 細(xì)胞膜受體

蛋白分子量  predicted molecular weight: 84kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TLR2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Toll樣受體2抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008].

Function : Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

Subunit : Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3.

Subcellular Location : Membrane; Single-pass type I membrane protein.

Tissue Specificity : Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

Post-translational modifications : Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

Similarity : Belongs to the Toll-like receptor family.

Contains 14 LRR (leucine-rich) repeats.

Contains 1 TIR domain.

Database links :

UniProtKB/Swiss-Prot: O60603

Entrez Gene: 448807 Dog

Entrez Gene: 7097 Human

Entrez Gene: 574290 Rhesus monkey

Omim: 603028 Human

SwissProt: Q95M53 Cynomolgus Monkey

SwissPot: Q689D1 Dog

SwissProt: O60603 Human

SwissProt: B3Y618 Rhesus monkey

Toll樣受體2（TLR2）通過(guò)激活天然免疫，參與特異性免疫應(yīng)答的啟動(dòng)，Toll樣受體2(TLR2)作為一種重要跨膜信號(hào)轉(zhuǎn)導(dǎo)受體參與了內(nèi)毒素誘發(fā)反應(yīng)的病理過(guò)程，對(duì)其調(diào)控機(jī)制的研究日益受到關(guān)注。

抗體的鑒定：

1）Toll樣受體2抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

IL-1 Beta(Interleukin-1 Beta) 白介素1β(白細(xì)胞介素-1β)(多肽抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-CD184/CXC-R4 細(xì)胞表面趨化因子受體4抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh PANK1 泛酸激酶1抗體 規(guī)格 0.2ml

HSPB1 Kit Human 人 HSP27 / HSPB1 ELISA配對(duì)抗體 ELISA

Transferrin 英文名稱(chēng)： 轉(zhuǎn)鐵蛋白/血清鐵傳遞蛋白抗體 0.1ml

Cdc25C 英文名稱(chēng)： 細(xì)胞分裂周期蛋白25C抗體 0.2ml

Anti-CD184/CXC-R4 細(xì)胞表面趨化因子受體4抗體Multi-class antibodies規(guī)格： 0.1ml

Big ET ELISA Kit 大鼠大內(nèi)皮素Multi-class antibodies規(guī)格： 48T

Anti-ATF2/FITC 熒光素標(biāo)記活化復(fù)制因子2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FAF1 Fas相關(guān)1因子抗體 規(guī)格 0.1ml

ACE(Mouse Angiotensin converting enzyme) ELISA Kit 小鼠緊張素轉(zhuǎn)化酶 96T

3-Mar 英文名稱(chēng)： 膜相關(guān)環(huán)指蛋白3抗體 0.2ml

ABCF2 英文名稱(chēng)： ATP結(jié)合盒轉(zhuǎn)運(yùn)蛋白2抗體 0.2ml

Anti-ATF2/FITC 熒光素標(biāo)記活化復(fù)制因子2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-VDCC-L alpha/FITC 熒光素標(biāo)記L-型電壓依賴型鈣通道α抗體IgGMulti-class antibodies規(guī)格： 0.2ml

CD56/NCAM1(Neural Cell Adhesion Molecule 1) 神經(jīng)細(xì)胞粘附分子1抗原Multi-class antibodies規(guī)格： 0.5mg

CD122/白介素2受體β鏈抗體 Anti-CD122/IL-2RB 0.1ml

STAT2 英文名稱(chēng)： 信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子2抗體 0.1ml

Exportin 1 英文名稱(chēng)： 染色體區(qū)域穩(wěn)定蛋白/核輸出蛋白1/染色體區(qū)域穩(wěn)定必需蛋白抗體 0.1ml

Rhesus antibody Rh phospho-GSK3 Alpha/Beta(Tyr279+Tyr216) 磷酸化葡萄糖合成激酶3α/β抗體 規(guī)格 0.1ml

CD56/NCAM1(Neural Cell Adhesion Molecule 1) 神經(jīng)細(xì)胞粘附分子1抗原Multi-class antibodies規(guī)格： 0.5mg

大鼠凝聚素(CLU)ELISA試劑盒 ，英文名： CLU ELISA Kit

人抗核抗體(ANA)ELISA檢測(cè)試劑盒HumanAi-nuclearAibody,ANAELISAKit 96T/48T

魚(yú)果糖1,6-二磷酸酶免疫試劑盒 Fish fructose-1,6-diphosphatase ELISA Kit

CLIAKitforPDGF-BBELISAKit大鼠血小板衍生生長(zhǎng)因子BB規(guī)格：48T/96T

噬菌體稀釋緩沖液500毫升

ELISAKitCFH人補(bǔ)體因子H規(guī)格：48T/96T

大鼠促腎上皮質(zhì)激素釋放激素(CRH)ELISA試劑盒 ，英文名： CRH ELISA Kit

Mouse 17 hydroxycoicosteroid (17-OHCS) ELISA Kit 小鼠17羥皮質(zhì)類(lèi)固醇(17-OHCS)ELISA試劑盒

ELISA 小鼠腦肽(mouse BNP) 48T/96T 進(jìn)口分裝

CLIAKitforLIFELISAKit大鼠抑制因子規(guī)格：48T/96T

通用型HCV(HEPATITISVIRUSC)病毒定量PCR擴(kuò)增檢測(cè)試劑盒20次

ELISAKitAflatoxin霉毒素規(guī)格：48T/96T

Toll樣受體2抗體規(guī)格 大鼠芳烴受體核轉(zhuǎn)位因子樣蛋白1(ARL)ELISA試劑盒 ，英文名： ARL ELISA Kit

Porcine soluble Vascuolar cell adhesion molecule 1 (sVCAM-1) ELISA Kit 豬可溶性細(xì)胞粘附分子1(sVCAM-1)ELISA試劑盒

牛特定基因序列(Bovine)核酸檢測(cè)試劑盒 48T

CLIAKitforMAGAb(Humanai-myelinassociatedglycoproteinaibody)ELISAKit人抗髓鞘相關(guān)糖蛋白抗體規(guī)格：48T/96T

體液(NO)活性熒光定量檢測(cè)試劑盒50次

ELISAKitEMP鹿紅細(xì)胞膜蛋白規(guī)格：48T/96T