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環(huán)指蛋白88抗體說(shuō)明書(shū) 英文名稱(chēng)  Anti-TRIM5α/RNF88

中文名稱(chēng)  環(huán)指蛋白88抗體說(shuō)明書(shū) 

別    名 TRIM5 alpha; RING finger protein 88; RNF88; TRIM5; TRIM5_HUMAN; tripartite motif protein 5; tripartite motif protein TRIM5; Tripartite motif-containing protein 5.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 54kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TRIM5α/RNF88 corresponding to amino acids 351-450.

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

環(huán)指蛋白88抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 TRIM5a is a 493 amino acid member of the large tripartite motif protein (TRIM) family. TRIM proteins are composed of three zinc-binding domains, a RING, a B-box 2 and a coiled-coil domain, and they use homomultimerization to identify different cell compartments. Some TRIM proteins, such as TRIM5? also possess a carboxy-terminal B30.2 (SPRY) domain and localize to the cytoplasm. TRIM5?mediates innate intracellular retroviral resistance, which is dependent on its carboxy-terminal domain. The three variable regions of the B30.2 domain form loops on one side of the B30.2 core structure of TRIM5?which may form a binding surface for the virus. TRIM5?trimerization plays a major role in its affinity for the retroviral capsid, and in its ability to inhibit virus infection. The linker region between the coiled-coil and B30.2 domains of TRIM5?is required for this trimerization. TRIM5?blocks infection after the virus has entered the cell.

Subunit : Isoform Alpha forms homotrimers, and may interact with retroviral CA protein. Isoform Delta interacts with BTBD1 and BTBD2.

Subcellular Location : Cytoplasm, P-body.

Similarity : Belongs to the TRIM/RBCC family.

Contains 1 B box-type zinc finger.

Contains 1 B30.2/SPRY domain.

Contains 1 RING-type zinc finger.

科學(xué)家經(jīng)過(guò)研究發(fā)現(xiàn)TRIM5蛋白有阻止HIV繁殖的作用，在毒感染過(guò)程中，一旦HIV進(jìn)入細(xì)胞，TRIM5蛋白立刻攔截并阻止其繁殖。因此， TRIM5是先天中對(duì)HIV的感應(yīng)器。與適應(yīng)性“后發(fā)制人”不同，先天能夠在接觸到病原體時(shí)立刻將其消除。

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