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| 產(chǎn)地 | 進口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11720 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-SynCAM/TSLC1 |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human TSLC1 C-terminus |
產(chǎn)品訂購信息：
英文名稱 Anti-SynCAM/TSLC1
中文名稱 細胞粘附分子1抗體說明書
別 名 CADM1; BL2; Cadm1; CADM1_HUMAN; Cell adhesion molecule 1; DKFZp686F1789; IGSF4; IGSF4A; Immunoglobulin Superfamily Member 4; MGC149785; MGC51880; NECL-2; NECL2; Nectin like 2; Nectin-like protein 2; RA175; SgIGSF; Spermatogenic immunoglobulin superfamily; ST17; sTSLC 1; Synaptic cell Adhesion Molecule; SynCAM; SynCAM1; TSLC-1; TSLC1; Tumor Suppressor in Lung Cancer 1.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog
產(chǎn)品類型 一抗
研究領(lǐng)域 免疫學(xué) 信號轉(zhuǎn)導(dǎo)
蛋白分子量 predicted molecular weight: 44kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TSLC1 C-terminus
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
細胞粘附分子1抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
Function : Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
Subunit : Homodimer. Interacts with CRTAM and EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts via its C-terminus with the PDZ domain of MPP3 and the PDZ domain of MPP6.
Subcellular Location : Cell membrane; Single-pass type I membrane protein. Note=Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder.
Similarity : Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Database links : UniProtKB/Swiss-Prot: Q9BY67.2
Entrez Gene: 23705 Human
Entrez Gene: 54725 Mouse
Entrez Gene: 363058 Rat
Omim: 605686 Human
SwissProt: Q9BY67 Human
SwissProt: Q8R5M8 Mouse
Unigene: 370510 Human
Unigene: 234832 Mouse
TSLC1屬于新的基因免疫球蛋白超家族, TSLC1蛋白質(zhì)參與細胞間黏附、細胞運動、信號轉(zhuǎn)導(dǎo)及. TSLC1在多種中表達異常,它對的影響主要表現(xiàn)為特性及基因表達,可抑制上皮間質(zhì)轉(zhuǎn)化.
TSLC1蛋白在控制生物侵襲性方面有著重要作用，也可能作為預(yù)測患者預(yù)后的重要生物標記，但是TSLC1的抑制分子機制還有待進一步研究。
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Recomb Protein A/Gold 金標記重組蛋白A(10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml
叉頭蛋白3-T細胞轉(zhuǎn)錄因子 Anti-FoxP3 0.1ml
Goat Anti-rabbit IgG/PE PE標記的羊抗兔IgG 0.1ml
FMO5 英文名稱： 二甲基苯胺單加氧酶5抗體 0.2ml
Rhesus antibody Rh phospho-STAT3 (Ser727) 磷酸化信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子3抗體 規(guī)格 0.1ml
Recomb Protein A/Gold 金標記重組蛋白A(10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml
IL-4(Interleukin-4) 白介素4抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-ADAMTS1 整合素樣金屬蛋白酶與凝血酶1型抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh phospho-Cdc6 (Ser54) 磷酸化細胞分裂周期蛋白6抗體 規(guī)格 0.1ml
免疫組化/原位雜交專用波片(雙面) 50片 真空車間清洗硅化處理,表面無污漬
Wnt8A 英文名稱： 信號通路WNT8A抗體 0.2ml
DUOXA2 英文名稱： 雙氧化酶激活因子2抗體 0.2ml
Anti-ADAMTS1 整合素樣金屬蛋白酶與凝血酶1型抗體Multi-class antibodies規(guī)格： 0.1ml
OJ/IleRS(Human anti-OJ-antibody) ELISA Kit 人OJ抗體/抗異亮氨酰tRNA合成酶抗體Multi-class antibodies規(guī)格： 48T
Anti-Phospho-PLC gamma 2 (Tyr1217) 磷酸化磷酯酶Cγ2抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh HHV8/ORF50 人類病毒8抗體 規(guī)格 0.2ml
ENA-78/CXCL5 ELISA Kit 大鼠上皮中性粒細胞活化肽78 96T
Classical swine fever virus 英文名稱： 抗體 0.2ml
C20orf26 英文名稱： 20號染色體開放閱讀框26抗體 0.2ml
Anti-Phospho-PLC gamma 2 (Tyr1217) 磷酸化磷酯酶Cγ2抗體Multi-class antibodies規(guī)格： 0.1ml
人白介素1可溶性受體Ⅱ(IL-1sRⅡ)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
兔子凝血因子Ⅱ(FⅡ)免疫試劑盒 Rabbit coagulation factor Ⅱ,FⅡ ELISA Kit
腎上腺髓質(zhì)素(ADM)ELISA試劑盒 ，英文名： ADM ELISA Kit
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Humanacidicfibroblastgrowthfactor1,aFGF-1ELISA試劑盒人酸性成纖維細胞生長因子1(aFGF-1)ELISA試劑盒規(guī)格：96T/48T
Humanai-Qfeveraibody,ai-Q-AbELISAKit人抗Q熱抗體(ai-Q-Ab)ELISA試劑盒規(guī)格：96T/48T
大鼠酸性磷酸酶(ACP)ELISA試劑盒 ，英文名： ACP ELISA Kit
人熱休克蛋白70(HSP-70)ELISA檢測試劑盒HumanHeatShockProtein70,HSP-70ELISAKit 96T/48T
鹿γ干擾素(IFNG)免疫試劑盒 Deer ierferon gamma,IFNG ELISA kit
CLIAKitforPR3Ab(HumanProteinase3Aibody)ELISAKit人蛋白酶3抗體規(guī)格：48T/96T
石蠟切片組織DAPKINASE蛋白表達熒光顯微鏡檢測試劑盒10/20次
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細胞粘附分子1抗體說明書 大鼠吻素受體(KISS1R)ELISA試劑盒 ，英文名： KISS1R ELISA Kit
Mouse dihydrotestosterone (DHT) ELISA Kit 小鼠雙氫睪酮(DHT)ELISA試劑盒
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抗體的生物素化標記實驗要點：
1. 細胞粘附分子1抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。
抗體的鑒定：
1）細胞粘附分子1抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
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