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★轉(zhuǎn)錄延伸調(diào)節(jié)蛋白1抗體說(shuō)明書(shū) 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

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★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

實(shí)驗(yàn)流程：

轉(zhuǎn)錄延伸調(diào)節(jié)蛋白1抗體說(shuō)明書(shū) 英文名稱(chēng)  Anti-TCERG1/CA150

中文名稱(chēng)  轉(zhuǎn)錄延伸調(diào)節(jié)蛋白1抗體說(shuō)明書(shū) 

別    名 CA 150; Co activator of 150 kDa; Coactivator of 150 kDa; MGC133200; TAF 2S; TAF2S; TATA box binding protein (TBP) associated factor RNA polymerase II S 150kD; TATA box binding protein associated factor 2S; TATA box binding protein associated factor RNA polymerase II S 150kD; TCERG 1; TCERG1; Transcription elongation regulator 1; Transcription factor CA 150; Transcription factor CA150; TCRG1_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/200μg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域     轉(zhuǎn)錄調(diào)節(jié)因子 細(xì)胞生物標(biāo)志物 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 124kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TCERG1/CA150

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

轉(zhuǎn)錄延伸調(diào)節(jié)蛋白1抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 CA150 is a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. It interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain.

Function : Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (-1) promoter.

Subunit : Binds formin (By similarity). Binds RNA polymerase II, HD and SF1.

Subcellular Location : Nucleus.

Tissue Specificity : Detected in brain neurons.

Similarity : Contains 6 FF domains.

Database links : UniProtKB/Swiss-Prot: O14776.2

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