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產(chǎn)品訂購信息：
英文名稱  Anti-TCTN1 

中文名稱   結(jié)構(gòu)蛋白家族1抗體說明書 

別    名  TCTN1; TECT1_HUMAN; Tectonic-1.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Cow, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 免疫學(xué) 神經(jīng)生物學(xué)

蛋白分子量  predicted molecular weight: 61kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from Human TCTN1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

結(jié)構(gòu)蛋白家族1抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Regulator of Hedgehog (Hh), required for both activation and inhibition of the Hh pathway in the patterning of the neural tube. During neural tube development, it is required for formation of the most ventral cell types and for full Hh pathway activation. Functions in Hh signal transduction to fully activate the pathway in the presence of high Hh levels and to repress the pathway in the absence of Hh signals. Modulates Hh signal transduction downstream of SMO and RAB23.

Function : Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Regulator of Hedgehog (Hh), required for both activation and inhibition of the Hh pathway in the patterning of the neural tube. During neural tube development, it is required for formation of the most ventral cell types and for full Hh pathway activation. Functions in Hh signal transduction to fully activate the pathway in the presence of high Hh levels and to repress the pathway in the absence of Hh signals. Modulates Hh signal transduction downstream of SMO and RAB23 (By similarity).

Subunit : Part of the tectonic-like complex (also named B9 complex) (By similarity).

Subcellular Location : Secreted.

DISEASE : Defects in TCTN1 are the cause of Joubert syndrome type 13 (JBTS13) [MIM:614173]. JBTS13 is a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease.

Similarity : Belongs to the tectonic family.

Database links : UniProtKB/Swiss-Prot: Q2MV58.2

Anti-STEAP1/FITC 熒光素標記跨膜上皮抗原1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

GIP (Gastric Inhibitory polypeptide) 胃泌素抑制肽(抗原)Multi-class antibodies規(guī)格： 0.5mg

人胎盤生長因子相關(guān)抗體 Anti-PLGF 0.1ml

Phospho-Stathmin (Ser16) 英文名稱： 磷酸化原癌基因蛋白18抗體 0.1ml

E-Selectin 英文名稱： E選擇素抗體 0.1ml

Rhesus antibody Rh Phospho-mTOR (Ser2481) 磷酸化雷帕霉素靶蛋白抗體 規(guī)格 0.1ml

GIP (Gastric Inhibitory polypeptide) 胃泌素抑制肽(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-SYVN1/FITC 熒光素標記滑膜細胞凋亡抑制物1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

IGFBP-5 ( Insulin-like Growth Factor Binding Protein 5、IBP5) 樣生長因子結(jié)合蛋白-5(抗原)Multi-class antibodies規(guī)格： 0.5mg

Ras相關(guān)區(qū)域家族1A抗體 Anti-RASSF1A 0.2ml

SVH 英文名稱： 蛋白剪接變異體蛋白抗體 0.2ml

EFHC2 英文名稱： EFHC2蛋白抗體 0.2ml

Rhesus antibody Rh phospho-MK5(Ser129) 磷酸化原活化蛋白激酶激酶5抗體 規(guī)格 0.1ml

IGFBP-5 ( Insulin-like Growth Factor Binding Protein 5、IBP5) 樣生長因子結(jié)合蛋白-5(抗原)Multi-class antibodies規(guī)格： 0.5mg

GABA/BSA γ1氨基偶聯(lián)血清白蛋白Multi-class antibodies規(guī)格： 1mg

Anti-HER4/ErbB4 HER4抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh OAT-1/SLC22A6 陰離子轉(zhuǎn)運蛋白-1抗體 規(guī)格 0.1ml

HRP/Horseradish Peroxidase 辣根過氧化物酶(辣根酶) 10mg

TRP2 英文名稱： 酪酸酶相關(guān)蛋白2抗體 0.2ml

c-Maf 英文名稱： 原癌基因cMAF抗體 0.2ml

Anti-HER4/ErbB4 HER4抗體Multi-class antibodies規(guī)格： 0.2ml

HGF Protein Rat 重組大鼠 HGF / Hepatocyte Growth Factor 蛋白

KYSE150 食管鱗癌

SCGB1A1 Others Mouse 小鼠 SCGB1A1 / uteroglobin 人細胞裂解液 (陽性對照)

ME-180人子宮頸表皮癌細胞 ME-180 human cervical epidermoid carcinoma cells McCOY's 5A+10%FBS

大鼠腎小球系膜細胞;HBZY-1

小鼠海馬趾星形膠質(zhì)細胞(MA-h)(5×105) kasumi-1, 人細胞株 Human

CL-0330COLO 320DM(人結(jié)直腸腺癌細胞)5×106cells/瓶×2

CLK3 Others Human 人 CLK3 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

人外根殼細胞總RNAHHORSC NA

CNE細胞，細胞 人胚,HFL-I細胞 原代骨骼肌細胞特制基礎(chǔ)無血清培養(yǎng)基Many types of cells包裝：500/100ml

豹貓肺成纖維樣細胞;LCL3

HA Others H5N1 甲型 H5N1 (A/Anhui/1/2005) 血凝素 (Hemagglutinin / HA) 人細胞裂解液 (陽性對照)

結(jié)構(gòu)蛋白家族1抗體說明書 HGF Protein Rat 重組大鼠 HGF / Hepatocyte Growth Factor 蛋白

KYSE150 食管鱗癌

SCGB1A1 Others Mouse 小鼠 SCGB1A1 / uteroglobin 人細胞裂解液 (陽性對照)

ME-180人子宮頸表皮癌細胞 ME-180 human cervical epidermoid carcinoma cells McCOY's 5A+10%FBS

大鼠腎小球系膜細胞;HBZY-1

小鼠海馬趾星形膠質(zhì)細胞(MA-h)(5×105) kasumi-1, 人細胞株 Human

抗體的生物素化標記實驗要點：

1. 結(jié)構(gòu)蛋白家族1抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

抗體的鑒定：

1）結(jié)構(gòu)蛋白家族1抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。