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| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11576 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-TGFB4/LEFTY2 |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human TGFB4 |
產(chǎn)品訂購信息：
英文名稱 Anti-TGFB4/LEFTY2
中文名稱 轉(zhuǎn)化生長因子β4抗體說明書
別 名 EBAF; Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily); Endometrial bleeding associated factor; Endometrial bleeding-associated factor; Left right determination factor 2; Left right determination factor A; Left-right determination factor 2; Left-right determination factor A; LEFTA; LEFTY 2; LEFTY2; LEFTYA; LFTY2_HUMAN; MGC46222; Protein lefty 2; Protein lefty A; Protein lefty-2; Protein lefty-A; Protein lefty2; PSEC0024; TGF beta 4; TGF-beta-4; TGFB4; Transforming growth factor beta 4; Transforming growth factor beta-4.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat
產(chǎn)品類型 一抗
研究領(lǐng)域 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 干細(xì)胞 生長因子和激素
蛋白分子量 predicted molecular weight: 32kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TGFB4
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
轉(zhuǎn)化生長因子β4抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 LEFTY2 is a member of the TGF-beta family of proteins. LEFTY2 is secreted and plays a role in left-right asymmetry determination of organ systems during development and may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene.
Function : Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
Subcellular Location : Secreted.
Tissue Specificity : Mesenchymal cells of the endometrial stroma.
Post-translational modifications : The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
DISEASE : Defects in LEFTY2 are the cause of left-right axis malformations (LRAM) [MIM:601877]. The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava.
Similarity : Belongs to the TGF-beta family.
Database links : UniProtKB/Swiss-Prot: O00292.2
Anti-Vimentin/FITC 熒光素標(biāo)記波形蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml
CGB(Chromogranin B) 嗜鉻粒素B抗原Multi-class antibodies規(guī)格： 0.5mg
周期素依賴激酶1抗體 Anti-Cdc2/P34CDC2/CDK1 0.1ml
SUFU/Suppressor of Fused 英文名稱： 抑制Hh信號通路蛋白SUFU抗體 0.2ml
ESAT6 英文名稱： 結(jié)核分枝桿菌分泌性蛋白ESAT6抗體 0.2ml
Rhesus antibody Rh phospho-GADD153 (Ser30) 磷酸化GADD153抗體 規(guī)格 0.1ml
CGB(Chromogranin B) 嗜鉻粒素B抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-SHP2/PTPN11/FITC 熒光素標(biāo)記蛋白酪氨酸磷酸酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml
ILK-1 (Integrin-linked protein kinase 1) 整合素連接激酶-1Multi-class antibodies規(guī)格： 0.5mg
P物質(zhì)受體抗體 Anti-NK-1/SuRCtance P Receptor 0.1ml
Smad4 英文名稱： Smad4抗體 0.1ml
EVA1 英文名稱： 髓鞘蛋白P0樣蛋白2抗體 0.2ml
Rhesus antibody Rh Phospho-p63 (Ser395) 磷酸化P63抑制基因抗體 規(guī)格 0.1ml
ILK-1 (Integrin-linked protein kinase 1) 整合素連接激酶-1Multi-class antibodies規(guī)格： 0.5mg
VASH1(Vasohibin 1) 兔抑制蛋白1抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-procaspase 3 半胱天冬酶-3酶原抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh PCNA [Proliferation Marker] 增殖細(xì)胞核抗原抗體 規(guī)格 0.1ml
GRN Kit Human 人 Granulin / GRN / Progranulin ELISA配對抗體 ELISA
TRPM1 英文名稱： 瞬時受體電位離子通道蛋白1抗體(M亞家族) 0.1ml
Cdc6 英文名稱： 細(xì)胞分裂周期蛋白6抗體 0.1ml
Anti-procaspase 3 半胱天冬酶-3酶原抗體Multi-class antibodies規(guī)格： 0.2ml
FAM3C Protein Human 重組人 FAM3C / ILEI 蛋白 (Fc 標(biāo)簽)
Ke-3 人細(xì)胞
ULBP2 Others Human 人 ULBP2 / N2DL-2 人細(xì)胞裂解液 (陽性對照)
MDA-MB-436人腺癌細(xì)胞 MDA-MB-436 human breast adenocarcinoma cells L-15培養(yǎng)基+10%FBS
中國倉鼠卵巢細(xì)胞K1(亞系克隆);CHO-K1
小鼠雪旺細(xì)胞(MSC)(5×105) MDA-MB-231, 人癌細(xì)胞 Human
CL-0338G1(發(fā)綠色熒光的小鼠胚胎干細(xì)胞)5×106cells/瓶×2
ESAM Others Mouse 小鼠 ESAM / Endothelial Cell Adhesion Molecule 人細(xì)胞裂解液 (陽性對照)
小鼠肺大動脈內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL
Eca-109人細(xì)胞 Human esophageal cancer cell line Eca-109. 1640+10% FBS
IL1B Protein Rat 重組大鼠 IL-1 beta / IL1B 蛋白 (mature form)
NCI-H524(人非小細(xì)胞細(xì)胞) 5×106cells/瓶×2 HUVEC(人臍內(nèi)皮細(xì)胞)
轉(zhuǎn)化生長因子β4抗體說明書 FAM3C Protein Human 重組人 FAM3C / ILEI 蛋白 (Fc 標(biāo)簽)
Ke-3 人細(xì)胞
ULBP2 Others Human 人 ULBP2 / N2DL-2 人細(xì)胞裂解液 (陽性對照)
MDA-MB-436人腺癌細(xì)胞 MDA-MB-436 human breast adenocarcinoma cells L-15培養(yǎng)基+10%FBS
中國倉鼠卵巢細(xì)胞K1(亞系克隆);CHO-K1
小鼠雪旺細(xì)胞(MSC)(5×105) MDA-MB-231, 人癌細(xì)胞 Human
抗體的生物素化標(biāo)記實驗要點：
1. 轉(zhuǎn)化生長因子β4抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）轉(zhuǎn)化生長因子β4抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
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