无码欧美成人av天堂456软件,大胆日本熟妇XXXX,9277在线观看免费播放,4p三个男人让我爽了一夜

上海莼試生物技術(shù)有限公司
   
菜單 Close 公司首頁(yè) 公司介紹 公司動(dòng)態(tài) 產(chǎn)品展廳 證書(shū)榮譽(yù) 聯(lián)系方式 在線留言
您當(dāng)前的位置: 網(wǎng)站首頁(yè) > 產(chǎn)品展廳 >抗體 >抑制基因Testin抗體說(shuō)明書(shū)
產(chǎn)品展廳
抑制基因Testin抗體說(shuō)明書(shū)
  • 品牌:上海莼試
  • 產(chǎn)地:進(jìn)口、國(guó)產(chǎn)
  • 貨號(hào):CS11552
  • 發(fā)布日期: 2019-01-18
  • 更新日期: 2025-04-23
產(chǎn)品詳請(qǐng)
產(chǎn)地 進(jìn)口、國(guó)產(chǎn)
品牌 上海莼試
保存條件 Store at -20 °C
貨號(hào) CS11552
應(yīng)用范圍 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
CAS編號(hào)
抗體名 Anti-Testin
克隆性
靶點(diǎn) 詳見(jiàn)說(shuō)明書(shū)
適應(yīng)物種 詳見(jiàn)說(shuō)明書(shū)
形態(tài) 詳見(jiàn)說(shuō)明書(shū)
宿主 詳見(jiàn)說(shuō)明書(shū)
亞型 IgG
標(biāo)識(shí)物 詳見(jiàn)說(shuō)明書(shū)
濃度 1mg/1ml%
免疫原 KLH conjugated synthetic peptide derived from human Testin (161-210aa)

產(chǎn)品訂購(gòu)信息:
英文名稱(chēng)  Anti-Testin 

中文名稱(chēng)   抑制基因Testin抗體說(shuō)明書(shū) 

      Testin; TES; TESS 2; TESS; TESS2; TESS-2; Testis derived transcript (3 LIM domains); Testis derived transcript; TES_HUMAN.


       1mg/1ml

規(guī)   0.2ml/100μg

抗體來(lái)源   Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Mouse, Rat, Dog, Pig, Rabbit, Sheep 

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域     信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 48kDa

       Lyophilized or Liquid

 KLH conjugated synthetic peptide derived from human Testin (161-210aa) 

       IgG

純化方法   affinity purified by Protein A

儲(chǔ)    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

抑制基因Testin抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

(石蠟切片需做抗原修復(fù)) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Cancer-associated chromosomal changes often involveregions containing fragile sites. This gene maps to a commomfragile site on chromosome 7q31.2 designated FRA7G. This gene issimilar to mouse Testin, a testosterone-responsive gene encoding aSertoli cell secretory protein containing three LIM domains. LIMdomains are double zinc-finger motifs that mediate protein-proteininteractions between transcription factors, cytoskeletal proteinsand signaling proteins. This protein is a negative regulator ofcell growth and may act as a tumor suppressor. This scaffoldprotein may also play a role in cell adhesion, cell spreading andin the reorganization of the actin cytoskeleton. Multiple proteinisoforms are encoded by transcript variants of this gene.

Function : Scaffold protein that may play a role in cell adhesion,cell spreading and in the reorganization of the actin cytoskeleton.Plays a role in the regulation of cell proliferation. May act as atumor suppressor. Inhibits tumor cell growth.

Subunit : Interacts via LIM domain 1 with ZYX. Interacts (via LIMdomain 3) with ENAH and VASP. Interacts with talin, actin,alpha-actinin, GRIP1 and PXN. Interacts (via LIM domain 2) withACTL7A (via N-terminus). Heterodimer with ACTL7A; the heterodimerinteracts with ENAH to form a heterotrimer.

Subcellular Location : Cytoplasm. Cell junction, focal adhesion.Note=Detected along actin stress fibers.

Tissue Specificity : Ubiquitous.

Similarity : Belongs to the prickle / espinas / testin family.

Contains 3 LIM zinc-binding domains.

Contains 1 PET domain.

Database links : UniProtKB/Swiss-Prot: Q9UGI8.1

Anti-TNF- Alpha /RBITC 紅色熒光素標(biāo)記壞死因子抗體Multi-class antibodies規(guī)格: 0.2ml

BMPR-1A(Bone Morphogenetic protein Receptor-1A) 骨成型蛋白受體1A抗原Multi-class antibodies規(guī)格: 0.5mg

壞死因子-β抗體 Anti-TNF-β 0.1ml

SAPAP3 英文名稱(chēng): PSD95結(jié)合蛋白3抗體 0.2ml

ERC1 英文名稱(chēng): RAB6蛋白抗體 0.2ml

Rhesus antibody Rh Phospho-Jak3 (Tyr785) 磷酸化蛋白酪氨酸激酶JAK-3抗體 規(guī)格 0.1ml

BMPR-1A(Bone Morphogenetic protein Receptor-1A) 骨成型蛋白受體1A抗原Multi-class antibodies規(guī)格: 0.5mg

Anti-Telomerase Binding Protein,P23/FITC 熒光素標(biāo)記端粒酶結(jié)合蛋白P23抗體IgGMulti-class antibodies規(guī)格: 0.2ml

M2-PK ( pyruvate Kinase M2) 酸激酶-M2(抗原)Multi-class antibodies規(guī)格: 0.5mg

抗豬病大腸桿菌志賀樣毒素Ⅱ型突變體抗體 Shiga-like toxin IIe variant subunit A[Escherichia coli] 0.2ml

phospho-STAT6 (Tyr641) 英文名稱(chēng): 磷酸化信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子6抗體 0.1ml

EYA4 英文名稱(chēng): EYA4蛋白抗體 0.2ml

Rhesus antibody Rh phospho-M-CSF Receptor(Tyr809) 磷酸化巨噬細(xì)胞集落刺激因子受體抗體 規(guī)格 0.1ml

M2-PK ( pyruvate Kinase M2) 酸激酶-M2(抗原)Multi-class antibodies規(guī)格: 0.5mg

GLP-1(human) 人胰素樣肽1Multi-class antibodies規(guī)格: 48T

Anti-Collagen X Ⅹ型膠原抗體Multi-class antibodies規(guī)格: 0.1ml

Rhesus antibody Rh Nogo R 軸索過(guò)度生長(zhǎng)抑制因子受體/Nogo受體抗體 規(guī)格 0.1ml

Human Alpha Lactalbumin, Alpha-La ELISA Kit 人α乳清蛋白 96T

TSHB(2T11) 英文名稱(chēng): 促甲狀腺素單克隆抗體 0.1ml

CD93 英文名稱(chēng): CD93抗體 0.2ml

Anti-Collagen X Ⅹ型膠原抗體Multi-class antibodies規(guī)格: 0.1ml

VEGFA Protein Canine 重組狗 VEGF / VEGFA 蛋白

Hep-2 人喉表皮癌細(xì)胞

OSM Others Human 人 Oncostatin M / OSM 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

T24人膀胱移行細(xì)胞癌細(xì)胞 T24 human bladder ansitional cell carcinoma cell 1640+10% FBS

非洲綠猴腎細(xì)胞系/HCV-NS5B;Vero-HCV-NS5B

大鼠少突膠質(zhì)前體細(xì)胞(ROPC)(5×105) SKOV3 [SK-OV-3], 人腺癌細(xì)胞系 Human

CL-0387MS751(人子宮頸表皮癌細(xì)胞)5×106cells/瓶×2

S100A6 Others Human 人 S100A6 / CACY 桿狀病毒-昆蟲(chóng)細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人虹膜色素上皮細(xì)胞總RNAHIPEpiC NA

WI-38細(xì)胞,人二倍體胚肺細(xì)胞 人,LTEP-a-2細(xì)胞 QBC-939, 人細(xì)胞

貓腎細(xì)胞;CRFK

NA Others H1N1 甲型 H1N1 (A/California/04/2009) 神經(jīng)氨酸酶 (Neuraminidase/NA) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

抑制基因Testin抗體說(shuō)明書(shū) VEGFA Protein Canine 重組狗 VEGF / VEGFA 蛋白

Hep-2 人喉表皮癌細(xì)胞

OSM Others Human 人 Oncostatin M / OSM 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

T24人膀胱移行細(xì)胞癌細(xì)胞 T24 human bladder ansitional cell carcinoma cell 1640+10% FBS

非洲綠猴腎細(xì)胞系/HCV-NS5B;Vero-HCV-NS5B

大鼠少突膠質(zhì)前體細(xì)胞(ROPC)(5×105) SKOV3 [SK-OV-3], 人腺癌細(xì)胞系 Human

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):

1. 抑制基因Testin抗體說(shuō)明書(shū) 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件;

3.NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;

5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定:

1抑制基因Testin抗體說(shuō)明書(shū) 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

 


聯(lián)系方式
手機(jī):13585831301
Q Q: