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產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS11530 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-TIM4 |
克隆性 | |
靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
宿主 | 詳見(jiàn)說(shuō)明書(shū) |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human TIM4 |
產(chǎn)品訂購(gòu)信息:
英文名稱(chēng) Anti-TIM4
中文名稱(chēng) T淋巴細(xì)胞膜蛋白4抗體規(guī)格
別 名 SMUCKLER; Spleen, mucin-containing, knockout of lymphotoxin protein; T cell immunoglobulin and mucin domain containing protein 4; T-cell immunoglobulin and mucin domain containing 4; T-cell membrane protein 4; TIM-4; Tim4; Tim 4; TIMD 4.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類(lèi)型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Pig, Cow, Horse, Rabbit
產(chǎn)品類(lèi)型 一抗
研究領(lǐng)域 免疫學(xué) t-淋巴細(xì)胞
蛋白分子量 predicted molecular weight: 42kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TIM4
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
T淋巴細(xì)胞膜蛋白4抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. Studies have shown that TIM 4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM 1, and that this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM 4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM 4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM 1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.
Anti-TFPI-2/FITC 熒光素標(biāo)記組織因子途徑抑制劑-2抗體IgGMulti-class antibodies規(guī)格: 0.2ml
ASPP2(apoptosis stimulating protein of P53 2) P53凋亡刺激蛋白2抗原Multi-class antibodies規(guī)格: 0.5mg
突觸相關(guān)膜蛋白25抗體 Anti-SNAP-25 0.1ml
srGAP3 英文名稱(chēng): 相關(guān)GAP蛋白抗體 0.1ml
epithelial Sodium Channel gamma 英文名稱(chēng): 上皮離子通道蛋白γ/γENaC抗體 0.2ml
Rhesus antibody Rh phospho-MAPKAPK5(Ser93) 磷酸化p38調(diào)節(jié)/激活蛋白激酶抗體 規(guī)格 0.1ml
ASPP2(apoptosis stimulating protein of P53 2) P53凋亡刺激蛋白2抗原Multi-class antibodies規(guī)格: 0.5mg
Anti-TGF- Beta1/RBITC 紅色熒光素羅丹明標(biāo)記TGF-β1蛋白抗體IgGMulti-class antibodies規(guī)格: 0.2ml
MOG35-55 (myelin oligo-dendrocyte glycoprotein-MOG) 髓鞘少樹(shù)突膠質(zhì)細(xì)胞糖蛋白(抗原)Multi-class antibodies規(guī)格: 1mg
粘膜選址素抗體 Anti-MADCAM-1 0.2ml
SRGAP1 英文名稱(chēng): Rho GTP酶激活蛋白13抗體 0.2ml
FRMD2 英文名稱(chēng): FRMD2蛋白抗體 0.2ml
Rhesus antibody Rh Phospho-PKC alpha/beta II (Thr638/641) 磷酸化蛋白激酶C α/β2抗體 規(guī)格 0.1ml
MOG35-55 (myelin oligo-dendrocyte glycoprotein-MOG) 髓鞘少樹(shù)突膠質(zhì)細(xì)胞糖蛋白(抗原)Multi-class antibodies規(guī)格: 1mg
IL-22 人白介素-22Multi-class antibodies規(guī)格: 48T
Anti-ChRM1 毒蕈堿型乙酰膽堿受體M1抗體Multi-class antibodies規(guī)格: 0.1ml
Rhesus antibody Rh Nuclear Matrix Protein p84/NMP84 核基質(zhì)p84蛋白抗體 規(guī)格 0.2ml
RNA wait RNA高效保存液 50ml
TSPAN4 英文名稱(chēng): 四分子交聯(lián)體4抗體(四旋蛋白) 0.2ml
CROT 英文名稱(chēng): 肉堿氧位甲基轉(zhuǎn)移酶抗體 0.2ml
Anti-ChRM1 毒蕈堿型乙酰膽堿受體M1抗體Multi-class antibodies規(guī)格: 0.1ml
TNFSF10 Protein Cynomolgus 重組食蟹猴 TNFSF10 / AIL / APO-2L 蛋白
GLC-82 肺腺癌
PGF Others Mouse 小鼠 PIGF / PLGF 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
HCT-15人結(jié)直腸腺癌細(xì)胞 HCT-15 human colorectal adenocarcinoma cells RPMI-1640(GIBCO)+10%FBS
EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞;KMY0907
人骨髓間充質(zhì)干細(xì)胞(骨髓)(HMSC-bm)(5×105) MGC80-3(MGC-803)人細(xì)胞 Human
CL-0416Psi2 DAP(小鼠胚胎成纖維細(xì)胞)5×106cells/瓶×2
VTCN1 Others Human 人 B7-H4 / B7S1 / B7x 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人表皮黑色素細(xì)胞-中色素RNAHEM-m miRNA5 μg
MCF-7(NEW)細(xì)胞,人癌細(xì)胞 小鼠雜交瘤細(xì)胞,ST2/o細(xì)胞 HET-1A, 人食管上皮細(xì)胞
MDA-MB-435(人癌高轉(zhuǎn)移細(xì)胞) 5×106cells/瓶×2
HFDPC Pellet 人卵泡毛細(xì)胞團(tuán)塊 > 1 mio.cells 成纖維細(xì)胞培養(yǎng)基FM
T淋巴細(xì)胞膜蛋白4抗體規(guī)格 TNFSF10 Protein Cynomolgus 重組食蟹猴 TNFSF10 / AIL / APO-2L 蛋白
GLC-82 肺腺癌
PGF Others Mouse 小鼠 PIGF / PLGF 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
HCT-15人結(jié)直腸腺癌細(xì)胞 HCT-15 human colorectal adenocarcinoma cells RPMI-1640(GIBCO)+10%FBS
EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞;KMY0907
人骨髓間充質(zhì)干細(xì)胞(骨髓)(HMSC-bm)(5×105) MGC80-3(MGC-803)人細(xì)胞 Human
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. T淋巴細(xì)胞膜蛋白4抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件;
3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)T淋巴細(xì)胞膜蛋白4抗體規(guī)格 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。