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| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS11295 |
| 應(yīng)用范圍 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-Supervillin |
| 克隆性 | |
| 靶點(diǎn) | 詳見(jiàn)說(shuō)明書 |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書 |
| 形態(tài) | 詳見(jiàn)說(shuō)明書 |
| 宿主 | 詳見(jiàn)說(shuō)明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human Supervillin |
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 監(jiān)理蛋白抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；
3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
產(chǎn)品訂購(gòu)信息：
英文名稱 Anti-Supervillin
中文名稱 監(jiān)理蛋白抗體品牌
別 名 Archvillin; p205/p250; SVIL_HUMAN.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué)
蛋白分子量 predicted molecular weight: 248kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Supervillin
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
監(jiān)理蛋白抗體品牌 產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Supervillin is an F-actin-binding protein originally with two reported isoforms of 205/250 kDa. The longer isoform, named archvillin, is muscle-specific. Supervillin is a tightly bound peripheral membrane protein that is concentrated at sites of epithelial cell-cell adhesion. It contributes to cell-cell adhesion, motility regulation, and information transfer between cell compartments. The COOH-terminus of supervillin is homologous to villin/gelsolin but is not responsible for the tight binding to the actin cytoskeleton in vivo. The NH2-terminus contains functional nuclear localization sequences and F-actin and myosin II binding domains. It has been suggested that supervillin may mediate actin and myosin II filament organization at cholesterol-rich membrane domains. Supervillin has been identified as a transcriptional activator of the androgen receptor. Supervillin is found in many cells of several species, but is most abundant in muscle, bone marrow, thyroid gland, and salivary gland.
Function : Forms a high-affinity link between the actin cytoskeleton and the membrane. Isoform 1 (archvillin) is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adehesions involves binding to TRIP6 (By similarity).
Subunit : Associates with F-actin. Interacts with NEB. Interacts with MYH9. Interacts with MYLK. Isoform 2 interacts with TRIP6. Isoform 2 interacts with DYNLT1 (By similarity).
Subcellular Location : Cell membrane; peripheral membrane protein; cytoplasmic side. Cytoplasm; cytoskeleton.
Tissue Specificity : Expressed in many tissues. Most abundant in muscle, bone marrow, thyroid gland and salivary gland. Isoform 1 (archvillin) is muscle specific.
Similarity : Belongs to the villin/gelsolin family.
Contains 5 gelsolin-like repeats.
Contains 1 HP (headpiece) domain.
Database links : UniProtKB/Swiss-Prot: O95425.2
抗體的鑒定：
1）監(jiān)理蛋白抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
ROCK2 (Rho-associated coiled-coil containing protein kinase 2) Rho相關(guān)蛋白激酶2抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-Phospho-Bad(Ser136) 磷酸化相關(guān)死亡促進(jìn)因子抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh phgophs-ATG4C(Ser451) 磷酸化自噬相關(guān)蛋白4C抗體 規(guī)格 0.1ml
Kappa Light Chain 濃縮液 0.1ml 進(jìn)口分裝
Urokinase 英文名稱： 尿激酶型纖溶酶原激活因子抗體 0.1ml
C4b-A 英文名稱： 補(bǔ)體C4b-A蛋白抗體 0.1ml
Anti-Phospho-Bad(Ser136) 磷酸化相關(guān)死亡促進(jìn)因子抗體Multi-class antibodies規(guī)格： 0.1ml
Anti-PPAR-delta /FITC 熒光素標(biāo)記D型-過(guò)氧化酶活化增生受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-Biotin Whole serum 兔抗生物素抗血清Multi-class antibodies規(guī)格： 1ml
干擾素-γ抗體 Anti-IFN-γAnti-Human 0.1ml
Donkey Anti-Goat IgG 驢抗羊IgG 1mg
FAM48A 英文名稱： P38相互作用蛋白抗體 0.1ml
Rhesus antibody Rh phospho-RAD51(Tyr315) 磷酸化Rad51抗體 規(guī)格 0.1ml
Rabbit Anti-Biotin Whole serum 兔抗生物素抗血清Multi-class antibodies規(guī)格： 1ml
Phospho-Smad3 (Ser208) 英文名稱： 磷酸化細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)分子SMAD3抗體 0.1ml
Phospho-Ezrin (Tyr353) 英文名稱： 磷酸化埃茲蛋白抗體 0.1ml
三葉肽因子3抗體 Anti-TFF3 0.1ml
Anti-TLR2/CD282 /FITC 熒光素標(biāo)記Toll樣受體2抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-LEF1(Ser42) 磷酸化淋巴增強(qiáng)因子-1抗體 規(guī)格 0.1ml
phospha-Bcl-xL (pSer62) peptide (human, mo, rat, bovine, dog, pig, sheep, horse) 磷酸化(Ser62)Bcl-xL蛋白抗原Multi-class antibodies規(guī)格： 0.5mg
HL-60, 人早幼粒細(xì)胞
B16 小鼠細(xì)胞
FCGR3A Others Human 人 CD16a / FCGR3A 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
大鼠腎小球系膜細(xì)胞;HBZY-1
肝動(dòng)脈平滑肌細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
人細(xì)胞;SF17 大鼠肝竇內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL
293T, SV40轉(zhuǎn)化的人胚腎上皮細(xì)胞系
KLK11 Others Mouse 小鼠 KLK11 / Kallikrein-11 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
&CHAR(10)&
NIH-3T3 小鼠成纖維細(xì)胞 NIH-3T3 mouse fibroblast cells DMEM+10% FBS
IFNL1 Protein Human 重組人 IL-29 / Ierleukin-29 蛋白 (His 標(biāo)簽)
NCI-H1703(人肺鱗癌細(xì)胞) 5×106cells/瓶×2 PA317(成纖維細(xì)胞)
監(jiān)理蛋白抗體品牌 HL-60, 人早幼粒細(xì)胞
B16 小鼠細(xì)胞
FCGR3A Others Human 人 CD16a / FCGR3A 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
大鼠腎小球系膜細(xì)胞;HBZY-1
肝動(dòng)脈平滑肌細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
人細(xì)胞;SF17 大鼠肝竇內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL
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