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產(chǎn)地 | 進(jìn)口、國產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號 | CS11265 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
CAS編號 | |
抗體名 | Anti-SPAG6 |
克隆性 | |
靶點(diǎn) | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標(biāo)識物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human SPAG6 |
產(chǎn)品訂購信息:
英文名稱 Anti-SPAG6
中文名稱 精子相關(guān)抗原6抗體費(fèi)用
別 名 PF16; PF16 protein homolog; Protein PF16 homolog; Repro SA 1; Repro-SA-1; SPAG6; SPAG6_HUMAN; Sperm associated antigen 6; Sperm flagellar protein; Sperm-associated antigen 6.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Horse
產(chǎn)品類型 一抗
研究領(lǐng)域 發(fā)育生物學(xué) 干細(xì)胞
蛋白分子量 predicted molecular weight: 55kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SPAG6
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
精子相關(guān)抗原6抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011].
Function : Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.
Subunit : Interacts with SPAG16 and SPAG17 (By similarity).
Subcellular Location : Cytoplasm, cytoskeleton. Cell projection, cilium, flagellum. Note=Associated with microtubules. Detected on the sperm flagellum.
Tissue Specificity : Highly expressed in testis.
Similarity : Contains 8 ARM repeats.
Database links : UniProtKB/Swiss-Prot: O75602.1
MUC*C/Mucin *C(Gastric Mucin M1) 胃粘液素抗原Multi-class antibodies規(guī)格: 0.5mg
Anti-Annexin IV 膜粘連蛋白 A4抗體Multi-class antibodies規(guī)格: 0.1ml
Rhesus antibody Rh Phospho-Bcl-2(Ser70) 磷酸化Bcl-2抗體 規(guī)格 0.1ml
Streptavidin-HRP 0.1ml 進(jìn)口分裝
VAMP8 英文名稱: 囊泡相關(guān)膜蛋白8抗體 0.1ml
D-DIMER 英文名稱: 交聯(lián)纖維蛋白二聚體抗體 0.2ml
Anti-Annexin IV 膜粘連蛋白 A4抗體Multi-class antibodies規(guī)格: 0.1ml
Anti-phospho-P53 (Thr81)/FITC 熒光素標(biāo)記磷酸化抑制基因P53抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rabbit Anti-dog IgG/Biotin 生物素化兔抗狗IgGMulti-class antibodies規(guī)格: 0.3ml
環(huán)子孢子蛋白抗體 anti-CSP 1ml
Rabbit Anti-mouse IgM/Alexa Fluor 555 Alexa Fluor 555標(biāo)記的兔抗小鼠IgM 0.1ml
phospho-FGFR3 (Tyr724) 英文名稱: 磷酸化成纖維細(xì)胞生長因子受體3抗體 0.1ml
Rhesus antibody Rh phospho-YWHAE(Thr232) 磷酸化14-3-3E蛋白抗體 規(guī)格 0.1ml
Rabbit Anti-dog IgG/Biotin 生物素化兔抗狗IgGMulti-class antibodies規(guī)格: 0.3ml
SORCS1 英文名稱: 液泡蛋白分選受體SORCS抗體 0.2ml
EMSY 英文名稱: EMSY蛋白抗體 0.2ml
色氨酸羥化酶抗體 Anti-TPH 0.2ml
Anti-TPH /FITC 熒光素標(biāo)記色氨酸羥化酶抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh Phospho-Jak1 (Tyr1022/1023) 磷酸化蛋白質(zhì)酪氨酸激酶JAK-1抗體 規(guī)格 0.1ml
BOb-1(B-cell oct-binding protein 1) B細(xì)胞特異性轉(zhuǎn)錄因子抗原Multi-class antibodies規(guī)格: 0.5mg
SW 1990 [SW-1990, SW1990] , 人*癌細(xì)胞
MRC-5 人胚
FCGR3A Others Rat 大鼠 CD16a / FCGR3A 人細(xì)胞裂解液 (陽性對照)
恒河猴肺細(xì)胞;RM-L1
*導(dǎo)管上皮細(xì)胞Many types of cells包裝:5 × 105次方(1ml)
人細(xì)胞;LNCaP 大鼠肺大動脈平滑肌細(xì)胞完全培養(yǎng)基 100mL
Asp2人胚胎腎細(xì)胞轉(zhuǎn)化細(xì)胞;FC33
人腸微內(nèi)皮細(xì)胞(HIMEC)( 5×105 )
CL-0374KP-N-NS(人腎上腺神經(jīng)母細(xì)胞瘤細(xì)胞())5×106cells/瓶×2
人腦星形膠質(zhì)母細(xì)胞瘤;U-87 MG [U87MG;U87 MG] 大鼠軟骨細(xì)胞完全培養(yǎng)基 100mL
EL4 小鼠細(xì)胞
PRSS3 Others Human 人 ypsin-3 / PRSS3 人細(xì)胞裂解液 (陽性對照)
精子相關(guān)抗原6抗體費(fèi)用 SW 1990 [SW-1990, SW1990] , 人*癌細(xì)胞
MRC-5 人胚
FCGR3A Others Rat 大鼠 CD16a / FCGR3A 人細(xì)胞裂解液 (陽性對照)
恒河猴肺細(xì)胞;RM-L1
*導(dǎo)管上皮細(xì)胞Many types of cells包裝:5 × 105次方(1ml)
人細(xì)胞;LNCaP 大鼠肺大動脈平滑肌細(xì)胞完全培養(yǎng)基 100mL
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 精子相關(guān)抗原6抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)精子相關(guān)抗原6抗體費(fèi)用 抗體的效價鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力??贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。