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產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS11260 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-SOX7 |
克隆性 | |
靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
宿主 | 詳見(jiàn)說(shuō)明書(shū) |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human SOX7 (42-100aa) |
產(chǎn)品訂購(gòu)信息:
英文名稱 Anti-SOX7
中文名稱 轉(zhuǎn)錄因子SOX7抗體說(shuō)明書(shū)
別 名 MGC10895; SOX 7; SOX 7 transcription factor; sox7; SOX7 transcription factor; SOX7_HUMAN; SRY (sex determining region Y) box 7; SRY box 7; SRY sex determining region Y box 7; Transcription factor SOX 7; Transcription factor Sox-7; Transcription factor SOX7.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 發(fā)育生物學(xué) 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué)
蛋白分子量 predicted molecular weight: 42kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SOX7 (42-100aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
轉(zhuǎn)錄因子SOX7抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
Function : Transcriptional repressor. Binds to the DNA sequence 5'-AACAAT-3'.
Subunit : Interacts with CTNNB1/beta-catenin; this interaction may lead to the proteasomal degradation of active CTNNB1 and thus inhibition of Wnt/beta-catenin-stimulated transcription.
Subcellular Location : Nucleus.
Similarity : Contains 1 HMG box DNA-binding domain.
Contains 1 Sox C-terminal domain.
Database links : UniProtKB/Swiss-Prot: Q9BT81.1
MLH1(Mutl homolog l gene) 錯(cuò)配修復(fù)蛋白1抗原Multi-class antibodies規(guī)格: 0.5mg
Anti-Annexin III 膜粘連蛋白A3抗體Multi-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh Phospho-Bcl-2(Thr129) 磷酸化Bcl-2抗體 規(guī)格 0.1ml
Streptavidin-AP 0.1ml 進(jìn)口分裝
vars2 英文名稱: 纈酰-tRNA合成酶抗體 0.2ml
Drebrin 英文名稱: 腦發(fā)育調(diào)節(jié)蛋白抗體 0.2ml
Anti-Annexin III 膜粘連蛋白A3抗體Multi-class antibodies規(guī)格: 0.2ml
Anti-PRCP/FITC 熒光素標(biāo)記脯氨酰羧肽酶抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rabbit Anti-FITC Whole serum 兔抗熒光素抗血清Multi-class antibodies規(guī)格: 1ml
樣生長(zhǎng)因子-I抗體 Anti-IGF-I 0.1ml
Rabbit Anti-Bov IgG 兔抗IgG 1mg
FYB 英文名稱: Fyn結(jié)合蛋白抗體 0.2ml
Rhesus antibody Rh phospho-Rab24(Ser95) 磷酸化ras基因相關(guān)蛋白R(shí)ab24抗體 規(guī)格 0.1ml
Rabbit Anti-FITC Whole serum 兔抗熒光素抗血清Multi-class antibodies規(guī)格: 1ml
Sidekick 2 英文名稱: 細(xì)胞粘附分子伴侶蛋白2抗體 0.2ml
Engrailed 1 英文名稱: 同源盒蛋白轉(zhuǎn)錄因子EN1抗體 0.2ml
壞死因子受體相關(guān)因子2抗體 Anti-TRAF2 0.2ml
Anti-TP I /FITC 熒光素標(biāo)記拓普西異構(gòu)酶Ⅰ抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh phospho-ITGB3(Tyr773) 磷酸化整合素β3抗體 規(guī)格 0.1ml
BRCA1(Breast cancer susceptbility gene 1)(mouse rat) 癌易感基因1抗原(小鼠 大鼠)Multi-class antibodies規(guī)格: 0.5mg
SP2/0, 小鼠細(xì)胞
MAO EBV-轉(zhuǎn)化人淋巴細(xì)胞
CD46 Others Human 人 CD46 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
CM-H018人成纖維細(xì)胞完全培養(yǎng)基100mL
真皮成纖維細(xì)胞Many types of cells包裝:5 × 105次方(1ml)
人Wilms細(xì)胞;G401 大鼠氣管平滑肌細(xì)胞完全培養(yǎng)基 100mL
BRL 3A, 大鼠肝正常細(xì)胞
ATL1 Others Human 人 ATL1 / SPG3A / Atlastin-1 桿狀病毒-昆蟲(chóng)細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人胚;WI-38 [WI38]
CHO細(xì)胞,中國(guó)倉(cāng)鼠卵巢細(xì)胞 人大細(xì)胞細(xì)胞,NCI-H460細(xì)胞 CL-0412PC-12(大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞)5×106cells/瓶×2
中國(guó)倉(cāng)鼠卵巢細(xì)胞(亞系克隆); EPO-CHO-T
HRG Others Human 人 HPRG / HRG 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
轉(zhuǎn)錄因子SOX7抗體說(shuō)明書(shū) SP2/0, 小鼠細(xì)胞
MAO EBV-轉(zhuǎn)化人淋巴細(xì)胞
CD46 Others Human 人 CD46 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
CM-H018人成纖維細(xì)胞完全培養(yǎng)基100mL
真皮成纖維細(xì)胞Many types of cells包裝:5 × 105次方(1ml)
人Wilms細(xì)胞;G401 大鼠氣管平滑肌細(xì)胞完全培養(yǎng)基 100mL
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 轉(zhuǎn)錄因子SOX7抗體說(shuō)明書(shū) 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件;
3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)轉(zhuǎn)錄因子SOX7抗體說(shuō)明書(shū) 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。