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| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS11183 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-RICTOR |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human RICTOR |
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 雷帕霉素靶蛋白復(fù)合體2抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
產(chǎn)品訂購信息：
英文名稱 Anti-RICTOR
中文名稱 雷帕霉素靶蛋白復(fù)合體2抗體品牌
別 名 Rictor(Phospho-Thr1135); Rictor(Phospho-T1135); p-Rictor(Thr1135); p-Rictor(T1135); AVO3; AVO3 homolog; hAVO3; DKFZp686B11164; hAVO3; KIAA1999; Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae); mAVO3; MGC39830; PIA; Pianissimo; Rapamycin insensitive companion of mTOR; Rapamycin-insensitive companion of mTOR; rictor; RICTR; RICTR_HUMAN; RPTOR independent companion of MTOR complex 2; TORC2 specific protein AVO3..
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Guinea Pig, G
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 細(xì)胞周期蛋白
蛋白分子量 predicted molecular weight: 192kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human RICTOR
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
雷帕霉素靶蛋白復(fù)合體2抗體品牌 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008].
Function : Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Subunit : Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to MTOR and PRR5 within the TORC2 complex. May interact with PRR5L.
Post-translational modifications : Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
Similarity : Belongs to the pianissimo family.
Database links : NCBI Reference Sequence: NP_689969.2 UniProtKB/Swiss-Prot: Q6R327.1
抗體的鑒定：
1）雷帕霉素靶蛋白復(fù)合體2抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
mTOR(Mammalian target of rapamycin) 雷帕霉素靶蛋白Multi-class antibodies規(guī)格： 0.5mg
Anti-Annexin V 重組膜粘連蛋白-5(人)抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh phospho-BCAR1(Tyr410) 磷酸化癌抗雌激素耐藥蛋白1 規(guī)格 0.1ml
兔抗人 IgM/FITC 0.2ml 綠色熒光，Dako公司
VRK1 英文名稱： 痘病毒相關(guān)激酶1抗體(絲酸/蘇酸蛋白激酶VRK1) 0.1ml
DDX58 英文名稱： DDX58抗體 0.2ml
Anti-Annexin V 重組膜粘連蛋白-5(人)抗體Multi-class antibodies規(guī)格： 0.1ml
Anti-Phospho-Pin1 (Ser16)/FITC 熒光素標(biāo)記磷酸化肽基脯氨酞順反異構(gòu)酶Pinl抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-mouse IgG 兔抗小鼠IgG (親和層析純化)Multi-class antibodies規(guī)格： 1mg
促胃泌素釋放肽抗體 Anti-GRP 0.1ml
Dog IgM/PE PE標(biāo)記的兔抗犬IgM抗體 0.1ml
FAM151A 英文名稱： FAM151A蛋白抗體 0.2ml
Rhesus antibody Rh Phospho-SATB1 (Ser47) 磷酸化核基質(zhì)結(jié)合區(qū)結(jié)合蛋白1抗體 規(guī)格 0.1ml
Rabbit Anti-mouse IgG 兔抗小鼠IgG (親和層析純化)Multi-class antibodies規(guī)格： 1mg
phospho-NCF1 (Ser359) 英文名稱： 磷酸化嗜中性粒細(xì)胞胞漿因子1抗體 0.1ml
ERAB 英文名稱： 內(nèi)質(zhì)網(wǎng)Aβ相關(guān)結(jié)合蛋白抗體 0.1ml
組織蛋白酶E抗體 Anti-cath-E/CE 0.1ml
Anti-Urocortin 3/FITC 熒光素標(biāo)記尿皮質(zhì)素UCN3抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-HMGN1(Ser20+Ser24) 磷酸化高遷移率核小體結(jié)合蛋白1 規(guī)格 0.1ml
CCR-2A(C-C Chemokine receptor 2A) 細(xì)胞表面趨化因子受體2A抗原Multi-class antibodies規(guī)格： 0.5mg
MN-h, 小鼠海馬神經(jīng)元
3T3A31 小鼠胚胎成纖維細(xì)胞
IL1R1 Others Rat 大鼠 IL1R1 / CD121a 人細(xì)胞裂解液 (陽性對(duì)照)
CL-0238U-87 MG(人神經(jīng)細(xì)胞)5×106cells/瓶×2
人上皮細(xì)胞cDNAHBEpiC cDNA
表達(dá)SV40T和EBNA1的人胚腎細(xì)胞;293ET 人腎上皮細(xì)胞完全培養(yǎng)基 100mL
CL-0143Lncap clone FGC(人細(xì)胞)5×106cells/瓶×2
CTSZ Others Human 人 CTSZ 人細(xì)胞裂解液 (陽性對(duì)照)
小鼠肝動(dòng)脈平滑肌細(xì)胞完全培養(yǎng)基 100mL
K7M2 wt [K7M2-WT]小鼠成骨細(xì)胞 K7M2 wt [K7M2-WT] murine osteosarcoma cells DMEM培養(yǎng)基+10%FBS
IL6 Protein Cynomolgus 重組食蟹猴 / 恒河猴 IL6 / Ierleukin-6 蛋白
RL1(大鼠肺成纖維樣細(xì)胞) 5×106cells/瓶×2 JAR(胚絨毛癌細(xì)胞)
雷帕霉素靶蛋白復(fù)合體2抗體品牌 MN-h, 小鼠海馬神經(jīng)元
3T3A31 小鼠胚胎成纖維細(xì)胞
IL1R1 Others Rat 大鼠 IL1R1 / CD121a 人細(xì)胞裂解液 (陽性對(duì)照)
CL-0238U-87 MG(人神經(jīng)細(xì)胞)5×106cells/瓶×2
人上皮細(xì)胞cDNAHBEpiC cDNA
表達(dá)SV40T和EBNA1的人胚腎細(xì)胞;293ET 人腎上皮細(xì)胞完全培養(yǎng)基 100mL
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