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| 產地 | 進口、國產 |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11072 |
| 應用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-RAD50 |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human RAD50 (817-872aa) |
產品訂購信息：
英文名稱 Anti-RAD50
中文名稱 DNA修復蛋白Rad50抗體說明書
別 名 DNA repair protein RAD50; hRad50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50_HUMAN; Rad50l; Truncated RAD50 protein.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep
產品類型 一抗
研究領域 染色質和核信號 表觀遺傳學
蛋白分子量 predicted molecular weight: 154kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human RAD50 (817-872aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
DNA修復蛋白Rad50抗體說明書 產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Function : Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Subunit : Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis.
Subcellular Location : Nucleus. Chromosome telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
Tissue Specificity : Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR
DISEASE : Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
Similarity : Belongs to the SMC family. RAD50 subfamily.
Contains 1 zinc-hook domain.
Database links : UniProtKB/Swiss-Prot: Q92878.1
FOXM1/HFH 11 peptide 插頭蛋白M1抗原Multi-class antibodies規(guī)格： 0.5mg
CD27 CD27 / TNFRSF7 兔單抗 (PE) Human
Rhesus antibody Rh phospho-DNA PK/PRKDC(Thr2609) 磷酸化DNA依賴性蛋白激酶抗體 規(guī)格 0.1ml
自顯影X膠片(蛋白印跡用) 3"×5" Kodak
Zinc finger protein 754 英文名稱： 鋅指蛋白754抗體 0.1ml
DHX15 英文名稱： DHX15蛋白抗體 0.2ml
CD27 CD27 / TNFRSF7 兔單抗 (PE) Human
Anti-RhoA /FITC 熒光素標記RhoA抗體IgGMulti-class antibodies規(guī)格： 0.2ml
TP-5(Thymopentin) 胸腺五肽Multi-class antibodies規(guī)格： 0.5mg
原活化蛋白激酶激酶1 Anti-MAPKK1 0.1ml
Phospho-SHP2 (Tyr81) 英文名稱： 磷酸化蛋白酪酸磷酸酶2抗體 0.1ml
FBRSL1 英文名稱： 病毒X蛋白反轉錄蛋白9抗體 0.2ml
Rhesus antibody Rh Phospho-PIM1(Tyr218) 磷酸化前病毒整合位點蛋白1抗體 規(guī)格 0.1ml
TP-5(Thymopentin) 胸腺五肽Multi-class antibodies規(guī)格： 0.5mg
ZNF239 英文名稱： 鋅指蛋白239抗體 0.2ml
DP1 英文名稱： 轉錄因子DP1抗體 0.1ml
芳香烴受體抗體 Anti-AHR 0.1ml
Anti-CD99/E2 antigen/FITC 熒光素標記CD99抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-FAK(Tyr925) 磷酸化粘著斑激酶抗體 規(guī)格 0.1ml
CTBP1(C-terminal binding protein 1) C端結合蛋白1抗原Multi-class antibodies規(guī)格： 0.5mg
VEGFA Protein Rat 重組大鼠 VEGF164 / VEGFA 蛋白
HT-29 人細胞
IFNGR1 Others Mouse 小鼠 IFNGR1 / CD119 人細胞裂解液 (陽性對照)
U-118 MG人腦星形膠質母細胞瘤 U-118 MG of brain asocytic blastoma DMEM培養(yǎng)基+10%FBS
家豬皮膚細胞;SSC-S1
大鼠間骨髓間充質干細胞(RMSC-bm)(5×105) MCF-7 [MCF7], 人癌細胞系 Human
CL-0367IM-9(人外周血B淋巴細胞)5×106cells/瓶×2
CD58 Others Human 人 LFA-3 / CD58 人細胞裂解液 (陽性對照)
人平滑肌細胞總RNAHPSMC NA
MDA-MB-231細胞，人癌細胞 BALB/c小鼠ES細胞,B/c-ES細胞 BPH-1, 人細胞
NCI-H1975(人) 5×106cells/瓶×2
HDMEC adult Pellet 人真皮微內皮細胞團塊(成年捐獻者) > 1 mio.cells 小氣道上皮細胞培養(yǎng)基SAEpiCM-prf
DNA修復蛋白Rad50抗體說明書 VEGFA Protein Rat 重組大鼠 VEGF164 / VEGFA 蛋白
HT-29 人細胞
IFNGR1 Others Mouse 小鼠 IFNGR1 / CD119 人細胞裂解液 (陽性對照)
U-118 MG人腦星形膠質母細胞瘤 U-118 MG of brain asocytic blastoma DMEM培養(yǎng)基+10%FBS
家豬皮膚細胞;SSC-S1
大鼠間骨髓間充質干細胞(RMSC-bm)(5×105) MCF-7 [MCF7], 人癌細胞系 Human
抗體的生物素化標記實驗要點：
1. DNA修復蛋白Rad50抗體說明書 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；
7.交聯反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產生競爭作用；
8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。
抗體的鑒定：
1）DNA修復蛋白Rad50抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
