无码欧美成人av天堂456软件,大胆日本熟妇XXXX,9277在线观看免费播放,4p三个男人让我爽了一夜

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. Ras蛋白特異鳥嘌呤核苷酸釋放因子1抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-Rasgrp1 

中文名稱   Ras蛋白特異鳥嘌呤核苷酸釋放因子1抗體費(fèi)用 

別    名  Calcium and DAG-regulated guanine nucleotide exchange factor II; CALDAG-GEFI; CALDAG-GEFII; Guanine nucleotide exchange factor, calcium and DAG regulated, Rap1A; hRasGRP1; Ras activator RasGRP; RAS guanyl nucleotide releasing protein 1; RAS guanyl releasing protein 1 (calcium and DAG-regulated); RAS guanyl releasing protein 1; RASGRP; RASGRP1; GRP1_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域     神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 結(jié)合蛋白 G蛋白偶聯(lián)受體 G蛋白信號

蛋白分子量  predicted molecular weight: 90kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Rasgrp1 (165-210aa) 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

Ras蛋白特異鳥嘌呤核苷酸釋放因子1抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008].

Function : RasGRP1 is a Ras-specific exchange factor, which is activated by T-cell receptor (TCR) and promotes TCR-dependent positive selection of thymocytes. RasGRP1 is highly expressed on most T lymphocytic leukemias and is a common site of proviral insertion in retrovirus-induced murine T-cell lymphomas.

Subunit : Forms a signaling complex with DGKZ and HRAS. Interacts with F-actin. Interacts with SKAP1.

Subcellular Location : Cell Membrane, Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus. Found in membrane fraction. Relocalization to the cell membrane upon activation is F-actin-dependent. Translocates to the Golgi in response to phorbol ester or nerve growth factor.

Tissue Specificity : Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level).

DISEASE : Defects in RASGRP1 may contribute to susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=Aberrantly spliced isoforms and/or diminished levels of RASGRP1 are found in a cohort of SLE patients raising the possibility that dysregulation of this signaling protein contributes to the development of autoimmunity in a subset of SLE patients.

Similarity : Belongs to the RASGRP family.

Contains 2 EF-hand domains.

Contains 1 N-terminal Ras-GEF domain.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 Ras-GEF domain.

Database links : UniProtKB/Swiss-Prot: O95267.2

抗體的鑒定：

1）Ras蛋白特異鳥嘌呤核苷酸釋放因子1抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

GATA-4(GATA binding factor 4) GATA結(jié)合蛋白4抗原Multi-class antibodies規(guī)格： 0.5mg

EPHB3 EphB3 / HEK2 兔單抗 (PE) Mouse

Rhesus antibody Rh Phospho-DARPP32(Thr75) 磷酸化多巴胺/cAMP調(diào)節(jié)磷蛋白抗體 規(guī)格 0.1ml

非預(yù)染蛋白Marker分子量標(biāo)準(zhǔn)(14.4-116kDa) 2x1000μl/400次 MBI原裝

Zyxin 英文名稱： 斑聯(lián)蛋白抗體 0.2ml

DIO2 英文名稱： 脫酶2抗體 0.1ml

EPHB3 EphB3 / HEK2 兔單抗 (PE) Mouse

FABP(brain) (Fatty acid-binding protein brain) 腦型脂肪酸結(jié)合蛋白抗原Multi-class antibodies規(guī)格： 0.5mg

一種基因抗體 Anti-BECN1 0.1ml

Rhesus antibody Rh phospho-EGFR (Ser1026) 磷酸化表皮生長因子受體抗體 規(guī)格 0.1ml

優(yōu)等胎血清 100ml 四季青

ZNF195 英文名稱： 鋅指蛋白195抗體 0.2ml

DHHC-20 英文名稱： 鋅指結(jié)構(gòu)蛋白酶20抗體 0.2ml

一種基因抗體 Anti-BECN1 0.1ml

ZAN 英文名稱： 透明帶黏附蛋白ZAN抗體 0.2ml

DOCK1 英文名稱： 細(xì)胞質(zhì)分裂付出蛋白1抗體 0.1ml

磷酸化活化復(fù)制因子2抗體 Anti-phospho-ATF2 0.1ml

Anti-Annexin V/PE 熒光素PE標(biāo)記兔抗人、大、小鼠、Annexin V抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-EIF4B (Ser540) 磷酸化真核翻譯起始因子4B抗體 規(guī)格 0.1ml

phospho-eIF4E(pSer209)(phospho-Eukaryotic translation initiation factor 4E) 磷酸化eIF-4E(Ser209)抗原Multi-class antibodies規(guī)格： 0.5mg

TNFSF11 Protein Cynomolgus 重組食蟹猴 RANKL / OPGL / TNFSF11 蛋白 (Fc 標(biāo)簽)

caski

ERBB3 Others Rhesus 恒河猴 HER3 / ErbB3 人細(xì)胞裂解液 (陽性對照)

Hepa1-6-EGFP(CMV-GFP慢病毒構(gòu)建穩(wěn)定株)小鼠細(xì)胞 Hepa1-6-EGFP (CMV-GFP leiviral consuct stable sain) of hepatocellular carcinoma cells in mice DMEM+10% FBS

小鼠B淋巴細(xì)胞雜交瘤細(xì)胞;SH3

人主動脈平滑肌細(xì)胞 (HASMC)( 5×105 ) HK-2, 人腎皮質(zhì)近曲小管上皮細(xì)胞 Human

CL-0462UMR-106(大鼠細(xì)胞)5×106cells/瓶×2

HA Others H7N9 甲型 H7N9 (A/Shanghai/2/2013) 血凝素 (Hemagglutinin / HA) 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

人髓核細(xì)胞RNAHNPC miRNA5 μg

兔角膜前基質(zhì)層成纖維細(xì)胞;RCFBF 癌細(xì)胞，MCF7細(xì)胞 Cl.Ly1+2-/9細(xì)胞，小鼠T淋巴細(xì)胞

人臍內(nèi)皮細(xì)胞;HUV-EC-C

HA Others H5N1 甲型 H5N1 (A/goose/Guiyang/337/2006) 血凝素 (Hemagglutinin / HA) 人細(xì)胞裂解液 (陽性對照)

Ras蛋白特異鳥嘌呤核苷酸釋放因子1抗體費(fèi)用 TNFSF11 Protein Cynomolgus 重組食蟹猴 RANKL / OPGL / TNFSF11 蛋白 (Fc 標(biāo)簽)

caski

ERBB3 Others Rhesus 恒河猴 HER3 / ErbB3 人細(xì)胞裂解液 (陽性對照)

Hepa1-6-EGFP(CMV-GFP慢病毒構(gòu)建穩(wěn)定株)小鼠細(xì)胞 Hepa1-6-EGFP (CMV-GFP leiviral consuct stable sain) of hepatocellular carcinoma cells in mice DMEM+10% FBS

小鼠B淋巴細(xì)胞雜交瘤細(xì)胞;SH3

人主動脈平滑肌細(xì)胞 (HASMC)( 5×105 ) HK-2, 人腎皮質(zhì)近曲小管上皮細(xì)胞 Human