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產(chǎn)品訂購信息：
英文名稱  Anti-RGMA 

中文名稱   反義導向分子RGMA抗體品牌 

別    名  Repulsive guidance molecule A; RGM domain family member A; RGMA; RGMA_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Pig, Cow 

產(chǎn)品類型   一抗  

研究領域    細胞生物 發(fā)育生物學 神經(jīng)生物學 細胞表面分子

蛋白分子量  predicted molecular weight: 28kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Repulsive Guidance Molecule A 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

反義導向分子RGMA抗體品牌 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The repulsive guidance molecule (RGM) family of proteins are important in the guidance of growth cones of developing neurons. They are repulsive for a group of axons, those from the temporal half of the retina. RGM have been implicated in both axonal guidance and neural tube closure, but unlike ephrins, semaphorins, netrins and slits, no receptor mechanism for RGM activation has been defined. Dorsal root ganglion axons do not respond to RGM but neogenin (a netrin-binding protein which can function as an RGM receptor) expression can spur RGM responsiveness. The RGM proteins are attached to the membrane by a GPI-anchor. Two members of this family, RGMa and RGMb, are expressed in the nervous system. RGMc, also known as hemojuvelin, is a part of the signaling pathway activating hepcidin and works together with hepcidin to restrict iron absorption in the gut. Defects in the gene encoding for RGMc cause the autosomal recessive disorder juvenile hemochromatosis (JH).

Function : Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

Subunit : Interacts with NEO1, BMP2 and BMP4

Subcellular Location : Cell membrane; Lipid-anchor, GPI-anchor

Similarity : Belongs to the repulsive guidance molecule (RGM) family.

Anti-Tie2/FITC 熒光素標記上皮生長因子樣域酪氨酸激酶2IgGMulti-class antibodies規(guī)格： 0.2ml

ATF6(activating transcription factor 6) 活化轉錄因子6抗原Multi-class antibodies規(guī)格： 0.5mg

src原癌基因抗體 Anti-SRC 0.2ml

Styk1 英文名稱： 絲酸/蘇酸/酪酸激酶STYK1抗體 0.2ml

Phospho-Estrogen Receptor alpha (Ser167) 英文名稱： 磷酸化雌激素受體ER alpha 抗體 0.1ml

Rhesus antibody Rh phospho-MAP4(Ser1046) 磷酸化微管相關蛋白4抗體 規(guī)格 0.1ml

ATF6(activating transcription factor 6) 活化轉錄因子6抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-PARP/FITC 熒光素標記多腺苷二磷酸多聚酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Goat Anti-human IgA/FITC 熒光素標記羊抗人IgAMulti-class antibodies規(guī)格： 0.3ml

人巨細胞病毒PP65/CMV低基質磷脂蛋白抗體 Anti-anti-HCMV PP65/HHV5 PP65 0.1ml

Rabbit Anti-mouse IgG-Fc/PE-CY5 PE-CY5標記的兔抗小鼠IgG-Fc 0.1ml

FMNL1 英文名稱： 成蛋白相關蛋白1抗體 0.2ml

Rhesus antibody Rh Phospho-Tuberin/TSC2(Ser1418) 磷酸化性硬化蛋白抗體 規(guī)格 0.1ml

Goat Anti-human IgA/FITC 熒光素標記羊抗人IgAMulti-class antibodies規(guī)格： 0.3ml

Lymphotactin 小鼠淋巴細胞趨化因子Multi-class antibodies規(guī)格： 48T

Anti-FLASH/CASP8AP2 半胱氨酸蛋白酶8相關蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Mouse Anti-Rabbit IgM 小鼠抗兔IgM 規(guī)格 1mg

Alpha2-PI (Human Alpha2-plasmin inhititor) ELISA Kit 人α2纖溶酶抑制物 96T

Syntrophin-1+2+3 英文名稱： 互養(yǎng)蛋白1,2,3抗體 0.1ml

CCDC6 英文名稱： 卷曲螺旋結構域蛋白6抗體(狀編碼蛋白) 0.2ml

Anti-FLASH/CASP8AP2 半胱氨酸蛋白酶8相關蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

T250g用于霍亂弧菌的培養(yǎng)(SN標準)

K氏培養(yǎng)基 250g 用于果汁或濃縮果汁中脂環(huán)芽孢桿菌

EEM培養(yǎng)基 EEM medium 250 用于致病性大腸桿菌的增菌培養(yǎng)和腸桿菌的增菌培養(yǎng)(Japan方法)

Aliz-gal瓊脂l/瓶用于大腸菌群快速檢測incubationmediaAliz-gal瓊脂l/瓶用于大腸菌群快速檢測

平板計數(shù)瓊脂平板(9cm) 10個/包 國際標準平板計數(shù)瓊脂，含糖，用于細菌總數(shù)的測定

Mar

堿性蛋白胨水 Alkaline Peptone Water 用于霍亂弧菌選擇性增菌培養(yǎng)(SN標準)

小牛血清 Calf serum 200毫升 診斷試劑用

Schaedler肉湯250g/瓶用于厭氧菌的增菌incubationmediaSchaedler肉湯250g/瓶用于厭氧菌的增菌

pH7.3PBS緩沖液 250g PBS是Tris-HCl緩沖鹽溶液

反義導向分子RGMA抗體品牌 m-TGE肉湯 250(g)  incubation media m-TGE肉湯 250(g)

m-TEC瓊脂 250(g)  incubation media m-TEC瓊脂 250(g)

酵母粉瓊脂 250(g)  incubation media 酵母粉瓊脂 250(g)

液體沙氏培養(yǎng)基 250(g)  incubation media 液體沙氏培養(yǎng)基 250(g)

抗體的生物素化標記實驗要點：

1. 反義導向分子RGMA抗體品牌 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）反義導向分子RGMA抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。