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| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10929 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-phospho-ROCK1(Thr455/Ser456) |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455/Ser456 |
產(chǎn)品訂購信息：
英文名稱 Anti-phospho-ROCK1(Thr455/Ser456)
中文名稱 磷酸化Rho相關(guān)蛋白激酶1抗體費(fèi)用
別 名 p-ROCK1(Thr455/Ser456); p160 ROCK1; p160ROCK; Renal carcinoma antigen NY REN 35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; ROCK1_HUMAN.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Horse, Rabbit
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 158kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455/Ser456
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化Rho相關(guān)蛋白激酶1抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq].
Function : Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation.
Subunit : Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.
Subcellular Location : Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.
Tissue Specificity : Detected in blood platelets.
Post-translational modifications : Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.
Database links :
UniProtKB/Swiss-Prot: Q13464.1
Entrez Gene: 6093 Human
Entrez Gene: 19877 Mouse
Entrez Gene: 81762 Rat
Omim: 601702 Human
SwissProt: Q8MIT6 Cow
SwissProt: Q13464 Human
SwissProt: P70335 Mouse
SwissProt: Q63644 Rat
ROCK1也是絲/蘇氨基酸激酶的一種，可調(diào)節(jié)細(xì)胞因子，促進(jìn)和肌動(dòng)蛋白的形成,因此又稱：含有卷曲螺旋的蛋白激酶。目前主要用于浸潤(rùn)、轉(zhuǎn)移方面的研究。
Anti-TSP-1/THBS1/FITC 熒光素標(biāo)記血小板反應(yīng)蛋白/凝血酶敏感蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Trk B 神經(jīng)生長(zhǎng)因子受體的一種(抗原)Multi-class antibodies規(guī)格： 0.5mg
ZCWCC1抗體 Anti-ZCWCC1 0.2ml
Stanniocalcin 1 英文名稱： 斯鈣素抗體 0.1ml
Exportin 1/CRM1 英文名稱： 染色體區(qū)域穩(wěn)定蛋白/核輸出蛋白1抗體 0.1ml
Rhesus antibody Rh Phospho-IKK alpha/beta (Ser176/Ser180) 磷酸化KB抑制蛋白激酶α/β抗體 規(guī)格 0.1ml
Trk B 神經(jīng)生長(zhǎng)因子受體的一種(抗原)Multi-class antibodies規(guī)格： 0.5mg
Anti-PPAR alpha/FITC 熒光素標(biāo)記α型-過氧化酶活化增生受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-Bovine IgG Whole serum 兔抗IgG抗血清Multi-class antibodies規(guī)格： 1ml
干擾素-γ抗體 Anti-IFN-γAnti-mouse、rat 0.1ml
Goat Anti-Mouse IgG 羊抗小鼠IgG 1mg
Fragilis 英文名稱： 干擾素誘導(dǎo)蛋白15抗體 0.2ml
Rhesus antibody Rh phospho-RAD51(Thr310) 磷酸化Rad51抗體 規(guī)格 0.1ml
Rabbit Anti-Bovine IgG Whole serum 兔抗IgG抗血清Multi-class antibodies規(guī)格： 1ml
PTN(Human pleiotrophin) ELISA Kit 人多效生長(zhǎng)因子Multi-class antibodies規(guī)格： 48T
Anti-GAD65 谷氨酸脫羧酶-65抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh Mouse Anti-human IgG/Cy5 Cy5標(biāo)記的小鼠抗人IgG 規(guī)格 0.1ml
Alpha1 Beta-GP(Human Alpha1 Beta glycoprotein) ELISA Kit 人α1β糖蛋白 96T
sp-selectin 英文名稱： 可溶性P選擇素抗體 0.1ml
CELSR2 英文名稱： 表皮生長(zhǎng)因子樣蛋白2抗體 0.1ml
Anti-GAD65 谷氨酸脫羧酶-65抗體Multi-class antibodies規(guī)格： 0.1ml
胰酪胨大豆肉湯24052250g用于金黃色葡萄球菌的多管發(fā)酵法測(cè)定及增菌培養(yǎng)(GB/T4789.08)。
H Medium Broth 培養(yǎng)基 100g incubation media H Medium Broth 培養(yǎng)基 100g
雪白根霉 檢測(cè)用菌 支/瓶
營(yíng)養(yǎng)肉湯(GB/T20944.7標(biāo)準(zhǔn))250g一般細(xì)菌培養(yǎng)，轉(zhuǎn)種，復(fù)壯，增菌等，也可用于消毒效果檢測(cè)
營(yíng)養(yǎng)瓊脂 250g 細(xì)菌總數(shù)測(cè)定，細(xì)菌傳代培養(yǎng)
NystatinTestMedium
抗生素檢定培養(yǎng)基8號(hào) 250(g) incubation media 抗生素檢定培養(yǎng)基8號(hào) 250(g)
TerrificBroth 500g 用于提高大腸桿菌中質(zhì)粒DNA的成品得率Terrific broth is used with glycerol in cultivating rec...
去氧膽酸于incubationmedia去氧膽酸于
TSCAgarBase
磷酸化Rho相關(guān)蛋白激酶1抗體費(fèi)用 規(guī)格： 微生物快速檢測(cè)計(jì)數(shù)卡 用途：
高敏感度大腸菌群計(jì)數(shù)板 規(guī)格： 25片/袋 用途： 用于大腸菌群計(jì)數(shù)，24小時(shí)可確定大腸菌群數(shù)
大腸桿菌計(jì)數(shù)板 規(guī)格： 25片/袋 用途： 可同時(shí)進(jìn)行大腸桿菌和大腸菌群計(jì)數(shù)，24-48小時(shí)可確認(rèn)大腸桿菌。
菌落總數(shù)計(jì)數(shù)板 規(guī)格： 50片/袋 用途： 菌落總數(shù)檢驗(yàn)
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 磷酸化Rho相關(guān)蛋白激酶1抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）磷酸化Rho相關(guān)蛋白激酶1抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
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