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產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS10923 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-phospho-Ret/HSCR1(Tyr1062) |
克隆性 | |
靶點(diǎn) | 詳見說(shuō)明書 |
適應(yīng)物種 | 詳見說(shuō)明書 |
形態(tài) | 詳見說(shuō)明書 |
宿主 | 詳見說(shuō)明書 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見說(shuō)明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human Ret around the phosphorylation site of Tyr1062 |
全、新、優(yōu)、品、好四大特點(diǎn):
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磷酸化原癌基因酪氨酸蛋白激酶受體RET抗體品牌 英文名稱 Anti-phospho-Ret/HSCR1(Tyr1062)
中文名稱 磷酸化原癌基因酪氨酸蛋白激酶受體RET抗體品牌
別 名 Ret (phospho Y1062); p-Ret(phospho Y1062); C ret; Cadherin family member 12; CDHF 12; CDHF12; ELKS Fusion gene; HSCR 1; HSCR1; Hydroxyaryl protein kinase; MEN2A; MEN2B; MTC 1; MTC1; Multiple endocrine neoplasia and medullary thyroid carcinoma 1; Oncogene RET; Proto oncogene tyrosine protein kinase receptor ret; Proto-oncogene c-Ret; Proto-oncogene tyrosine-protein kinase receptor ret; PTC; RET; RET ELE1; Ret Proto oncogene; RET transforming sequence; RET_HUMAN; RET51.
產(chǎn)品屬性:
濃 度 1mg/1ml
規(guī) 格 0.1ml/200μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 生長(zhǎng)因子和激素 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 119kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Ret around the phosphorylation site of Tyr1062
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
磷酸化原癌基因酪氨酸蛋白激酶受體RET抗體品牌 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Probable receptor with tyrosine-protein kinase activity; important for development.
Subunit : Phosphorylated form interacts with the PBT domain of DOK2, DOK4 and DOK5. The phosphorylated form interacts with PLCG1 and GRB7 (By similarity). Interacts (not phosphorylated) with CC PTK2/FAK1 (via FERM domain). Extracellular cell-membrane anchored RET cadherin fragments form complex in neurons with reduced trophic status, preferentially at the contact sites between somas. Interacts with AIP in the pituitary gland; this interaction prevents the formation of the AIP-survivin complex. Binds to ARTN.
Subcellular Location : Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein.
Post-translational modifications : Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062.
Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons.
DISEASE : Defects in RET may be a cause of colorectal cancer (CRC) [MIM:114500].
Defects in RET are a cause of Hirschsprung disease type 1 (HSCR1) [MIM:142623]. HSCR1 is a disorder of neural crest development characterized by the absence of intramural ganglion cells in the myenteric and submucosal plexuses of the gastrointestinal tract, often resulting in intestinal obstruction. Total colonic aganglionosis and total intestinal Hirschsprung disease also occur. Occasionally, MEN2A or FMTC occur in association with HSCR1.
Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 cadherin domain.
Contains 1 protein kinase domain.
實(shí)驗(yàn)流程:
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技術(shù)外包服務(wù):
★磷酸化原癌基因酪氨酸蛋白激酶受體RET抗體品牌 分子生物學(xué):質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué):基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
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