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磷酸化蛋白激酶C亞性D型抗體費用
  • 品牌:上海莼試
  • 產(chǎn)地:進口、國產(chǎn)
  • 貨號:CS10805
  • 發(fā)布日期: 2019-01-04
  • 更新日期: 2025-04-30
產(chǎn)品詳請
產(chǎn)地 進口、國產(chǎn)
品牌 上海莼試
保存條件 Store at -20 °C
貨號 CS10805
應用范圍 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
CAS編號
抗體名 Anti-phospho-PKC delta (Ser645)
克隆性
靶點 詳見說明書
適應物種 詳見說明書
形態(tài) 詳見說明書
宿主 詳見說明書
亞型 IgG
標識物 詳見說明書
濃度 1mg/1ml%
免疫原 KLH conjugated Synthesised phosphopeptide derived from human PKC delta around the phosphorylation site of Ser645

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磷酸化蛋白激酶C亞性D型抗體費用 英文名稱  Anti-phospho-PKC delta (Ser645)

中文名稱  磷酸化蛋白激酶C亞性D型抗體費用 

     PRKCD(phospho S645); PKCδ(Phospho-Ser645); MAY 1; MAY1; nPKC delta; PCKd; PKC d; PKC delta; PKC-d; PKCD; PKCdelta; PRKC D; PRKC delta; PRKC-d; PRKCd; Protein Kinase C delta; Protein kinase C delta type; Protein Kinase Cdelta; KPCD_HUMAN.

產(chǎn)品屬性:

      1mg/1ml

規(guī)   0.1ml/100μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human

產(chǎn)品類型   一抗 磷酸化抗體  

研究領域     細胞生物 免疫學 信號轉(zhuǎn)導 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 77kDa 

       Lyophilized or Liquid

  KLH conjugated Synthesised phosphopeptide derived from human PKC delta around the phosphorylation site of Ser645

      IgG

純化方法   affinity purified by Protein A

   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化蛋白激酶C亞性D型抗體費用 產(chǎn)品應用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

(石蠟切片需做抗原修復) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis.

Function : Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Is highly expressed in a number of cancer cells and promotes cell survival and resistance against chemotherapeutic drugs by inducing cyclin D1 (CCND1) and hyperphosphorylation of RB1, and via several pro-survival pathways, including NF-kappa-B, AKT1 and MAPK1/3 (ERK1/2). Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.

Subunit : Interacts with PDK1 (via N-terminus region), RAD9A, CDCP1, MUC1 and VASP.

Subcellular Location : Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Endoplasmic reticulum. Mitochondrion. Cell membrane; Peripheral membrane protein.

Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: Q05655.2

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