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產(chǎn)品訂購信息：
英文名稱  Anti-PDE4A 

中文名稱   磷酸二酯酶4A抗體說明書 

別    名  5''-cyclic phosphodiesterase 4A; cAMP specific 3 5 cyclic phosphodiesterase 4A; cAMP specific phosphodiesterase; cAMP-specific 3''; cAMP-specific 3',5'-cyclic phosphodiesterase 4A; DPDE2; dunce like phosphodiesterase E2; PDE 4A; PDE4; PDE46; PDE4A; PDE4A_HUMAN; PDE4A11; Phosphodiesterase 4A; phosphodiesterase 4A cAMP specific; Phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog; Phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4; phosphodiesterase type 4A11.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    免疫學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 98kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PDE4A 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸二酯酶4A抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling. There are 5 isoforms.

Function : Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Subunit : Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Subcellular Location : Isoform 1: Cytoplasm, perinuclear region.

Isoform 4: Membrane; Peripheral membrane protein. Note=Isoform 4 has propensity for association with membranes.

Isoform 6: Cytoplasm, perinuclear region.

Isoform 7: Cytoplasm. Membrane. Note=Predominantly cytoplasmic.

Tissue Specificity : Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).

Post-translational modifications : Phosphorylation by MAPKAPK2 its activation through PKA phosphorylation (By similarity). Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.

Proteolytically cleaved by caspase-3.

DISEASE : Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).

Similarity : Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Database links : UniProtKB/Swiss-Prot: P27815.3

Anti-BCRP/ABCG2/BXP-21 /FITC 熒光素標(biāo)記三磷酸腺苷結(jié)合轉(zhuǎn)運蛋白G超家族成員2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-GLP-2/FITC 熒光素標(biāo)記胰素樣肽-2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CK8 細(xì)胞角蛋白8抗體 規(guī)格 0.1ml

E7 protein/HPV 16 (Human papillomavirus type 16) 人瘤病毒16型E7蛋白抗原 0.5mg

IFNK 英文名稱： 干擾素kappa蛋白抗體 0.2ml

Rhesus antibody Rh TRAILR2/DR5/CD262 細(xì)胞調(diào)亡素/死亡受體5抗體 規(guī)格 0.1ml

Anti-GLP-2/FITC 熒光素標(biāo)記胰素樣肽-2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

PDH E1(Human pyruvate dehydrogenase-E1) ELISA Kit 人酸脫氫酶E1Multi-class antibodies規(guī)格： 48T

Anti-Phospho-REV-ERB alpha (Ser55/59) 磷酸化細(xì)胞核受體Rev-Erbα抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh IGF II/IGF2 樣生長因子-II抗體 規(guī)格 0.1ml

ANA(Human Anti-nuclear Antibody) ELISA Kit 人抗核抗體 96T

PCNP 英文名稱： PEST含核蛋白抗體 0.2ml

CNG3 英文名稱： 視錐感光細(xì)胞環(huán)磷酸鳥苷門控通道蛋白CNG3抗體 0.2ml

Anti-Phospho-REV-ERB alpha (Ser55/59) 磷酸化細(xì)胞核受體Rev-Erbα抗體Multi-class antibodies規(guī)格： 0.1ml

human phospho-inhibitory subunit of NF-κB Alpha,pIKB Alpha ELISA kit 人磷酸化核因子κB抑制蛋白α(IKKα human ELISA kit)Multi-class antibodies規(guī)格： 96T

Anti-Phospho-SHIP2 (Tyr1135) 磷酸化蛋白酪氨酸磷酸酶2抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Goat Anti-rat IgG/PE-Cy7 PE-Cy7標(biāo)記的羊抗大鼠IgG 規(guī)格 0.1ml

MC Ab(Human melanocyte antibody) ELISA Kit 人黑色素細(xì)胞抗體 96T

phospho-NFATC4 (Ser168 + Ser170) 英文名稱： 磷酸化T細(xì)胞激活核轉(zhuǎn)錄因子4抗體 0.1ml

BEND3 英文名稱： BEND3蛋白抗體 0.2ml

Anti-Phospho-SHIP2 (Tyr1135) 磷酸化蛋白酪氨酸磷酸酶2抗體Multi-class antibodies規(guī)格： 0.1ml

CL-0044C2C12(小鼠成肌細(xì)胞)5×106cells/瓶×2

GPNMB Others Human 人 GPNMB / Osteoactivin 人細(xì)胞裂解液 (陽性對照)

肌細(xì)胞分離液MDS

OVAC細(xì)胞，人細(xì)胞 猴腎C細(xì)胞,M145細(xì)胞 視網(wǎng)膜微內(nèi)皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

EB (NBL-4) (牛胚氣管細(xì)胞) 5×106cells/瓶×2

HREpC Pellet 人類腎小管上皮細(xì)胞團塊 > 1 mio.cells 人心肌細(xì)胞cDNAHCM cDNA

CL-0042BxPC-3(人原位*腺癌細(xì)胞)5×106cells/瓶×2

IgG Others Human 人 IgG1 Fc CHO細(xì)胞裂解液 (陽性對照)

抗霉菌溶液ABAMS

大額牛肺細(xì)胞;BFR-L1 中國倉鼠卵巢細(xì)胞，CHO細(xì)胞 Lncap(細(xì)胞)

EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞;KM933

SERPINE1 Others Human 人 PAI-1 / SerpinE1 人細(xì)胞裂解液 (陽性對照)

磷酸二酯酶4A抗體說明書 KYNU Others Mouse 小鼠 KYNU / Kynureninase 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

人腦微內(nèi)皮細(xì)胞總RNAHBMEC NA

大鼠細(xì)胞;RH-35

SPC-A-1細(xì)胞， 人細(xì)胞,HB細(xì)胞 tTA基因修飾的小鼠(B類);P19-CAG-tTA-1D3

雜交瘤(B類);IB3C10H12A12G2H8F3

PVRL2 Others Mouse 小鼠 CD112 / PVRL2 人細(xì)胞裂解液 (陽性對照)

抗體的生物素化標(biāo)記實驗要點：

1. 磷酸二酯酶4A抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）磷酸二酯酶4A抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。